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Lookup NU author(s): Dr Onur Sen,
Professor Jonathan Higgins
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
SiR-Hoechst (SiR-DNA) is a far-red fluorescent DNA probe being used widely for time-lapse imaging of living cells that is reported to be minimally toxic at concentrations as high as 10 - 25 µM. However, measuring nuclear import of Cyclin B1, inhibition of mitotic entry, and the induction of gH2AX foci in cultured human cells reveals that SiR-Hoechst induces DNA damage responses and G2 arrest at concentrations well below 1 µM. SiR-Hoechst is useful for live cell imaging, but it should be used with caution and at the lowest practicable concentration.
Author(s): Sen O, Saurin AT, Higgins JMG
Publication type: Article
Publication status: Published
Journal: Scientific Reports
Online publication date: 21/05/2018
Acceptance date: 09/05/2018
Date deposited: 09/05/2018
ISSN (electronic): 2045-2322
Publisher: Nature Publishing Group
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