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Lookup NU author(s): Professor Konstantinos StellosORCiD
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© Copyright 2017 S. Karger AG. All rights reserved. Background/Aims: Platelets affect endothelial progenitor cell (EPC) functionality, inducing their differentiation into mature endothelial cells. However, it remains to be established whether EPCs can influence platelet functionality. Methods: Mononuclear proangiogenic cells (MPCs) and early outgrowth cells (EOCs) were prepared from peripheral blood mononuclear cells, whereas late-outgrowth endothelial cells (OECs) were prepared from cord blood CD34+ cells. The effect of the above cells and their supernatants on washed platelet aggregation was studied. The effect of OECs and their supernatant on the adenosine diphosphate (ADP)-induced magnitude of platelet integrin receptor αIIbβ3 activation and on P-selectin membrane expression was investigated. The levels of nitric oxide (NO) and prostacyclin (PGI2) that were secreted from EOCs, OECs, and human umbilical vein endothelial cells (HUVECs) were also assessed. Results: Among all progenitors, OECs and their supernatant exhibited the most potent inhibitory activity towards platelet aggregation. Furthermore, OECs and their supernatant, but not CD34+ cells, reduced αIIbβ3 activation and P-selectin membrane expression. Finally, OECs secreted higher NO and PGI2 levels than EOCs did. Conclusion: The anti-platelet effect of EPCs depends highly on the degree of their endothelial phenotype, with OECs expressing the highest potency. Therefore, the induction of OEC generation and the enhancement of their functionality in vivo could be a new approach for the treatment of patients at a high thrombotic risk.
Author(s): Chantzichristos VG, Gkrozou F, Stellos K, Paschopoulos ME, Tselepis AD
Publication type: Article
Publication status: Published
Journal: Journal of Vascular Research
Year: 2018
Volume: 55
Issue: 1
Pages: 13-25
Print publication date: 01/01/2018
Online publication date: 02/12/2017
Acceptance date: 23/09/2017
ISSN (print): 1018-1172
ISSN (electronic): 1423-0135
Publisher: S. Karger AG
URL: https://doi.org/10.1159/000481779
DOI: 10.1159/000481779
PubMed id: 29197873
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