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Blastococcus xanthinilyticus sp. Nov., isolated from monument

Lookup NU author(s): Dr Imen Nouioui, Professor Hans-Peter Klenk, Dr Maria Del Carmen Montero-CalasanzORCiD

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Abstract

© 2018 IUMS. A novel, non-motile, coccoid, Gram-stain-positive actinobacterium, designated BMG 862T, was isolated from a marble sample collected from the Bulla Regia monument, Northern Tunisia. Its taxonomic position was determined using a polyphasic approach. Results from chemotaxonomic analyses showed MK-9(H4), MK-8(H4) and MK-9(H2) as the predominant menaquinones. The major polar lipids comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, glycophosphatidylinositol, hydroxy-phosphatidylethanolamine and three unidentified phospholipids. The fatty acids consisted of significant amounts (≥10%) of iso-C16:0, C17:1ω8c, iso-C15:0 and C16:1ω7c. Phylogenetic analysis on the basis of 16S rRNA gene sequence comparisons showed that strain BMG 862T belongs to the genus Blastococcus, being most closely related to Blastococcus saxobsidens (=DSM 44509T) (99.5%) and Blastococcus capsensis (=DSM 46835T=CECT 8876T) (99.3%). The genomic DNA G+C content of the organism was 74.7 mol%. Results of DNA-DNA hybridization and physiological tests allowed differentiation of strain BMG 862T from related species. The strain was also characterized by its ability to hydrolyse xanthine. On the basis of phenotypic and molecular characteristics, strain BMG 862T (=DSM 46842T=CECT 8884T) represents the type strain of a novel species of the genus Blastococcus, for which the name Blastococcus xanthinilyticus sp. nov. is proposed.

Publication metadata

Author(s): Hezbri K, Nouioui I, Rohde M, Sproer C, Schumann P, Gtari M, Klenk H-P, Montero-Calasanz MDC, Ghodhbane-Gtari F

Publication type: Article

Publication status: Published

Journal: International Journal of Systematic and Evolutionary Microbiology

Year: 2018

Volume: 68

Issue: 4

Pages: 1177-1183

Print publication date: 01/04/2018

Online publication date: 19/02/2018

Acceptance date: 05/02/2018

ISSN (print): 1466-5026

ISSN (electronic): 1466-5034

Publisher: Microbiology Society

URL: https://doi.org/10.1099/ijsem.0.002646

DOI: 10.1099/ijsem.0.002646

PubMed id: 29458502

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