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Lookup NU author(s): Dr Imen Nouioui, Dr Maria Del Carmen Montero-CalasanzORCiD, Professor Alan Bull, Professor Michael Goodfellow
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
© 2018 A polyphasic study was undertaken to establish the taxonomic status of three representative Geodermatophilus strains isolated from an extreme hyper-arid Atacama Desert soil. The strains, isolates B12T, B20 and B25, were found to have chemotaxonomic and morphological properties characteristic of the genus Geodermatophilus. The isolates shared a broad range of chemotaxonomic, cultural and physiological features, formed a well-supported branch in the Geodermatophilus 16S rRNA gene tree in which they were most closely associated with the type strain of Geodermatophilus obscurus. They were distinguished from the latter by BOX-PCR fingerprint patterns and by chemotaxonomic and other phenotypic properties. Average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between the whole genome sequences of isolate B12T and G. obscurus DSM 43160T were 89.28%, 87.27% and 37.4%, respectively, metrics consistent with its classification as a separate species. On the basis of these data, it is proposed that the isolates be assigned to the genus Geodermatophilus as Geodermatophilus chilensis sp. nov. with isolate B12T (CECT 9483T = NCIMB 15089T) as the type strain. Analysis of the whole genome sequence of G. chilensis B12T with 5341 open reading frames and a genome size of 5.5 Mb highlighted genes and gene clusters that encode for properties relevant to its adaptation to extreme environmental conditions prevalent in extreme hyper-arid Atacama Desert soils.
Author(s): Castro JF, Nouioui I, Sangal V, Trujillo ME, Montero-Calasanz MDC, Rahmani T, Bull AT, Asenjo JA, Andrews BA, Goodfellow M
Publication type: Article
Publication status: Published
Journal: Systematic and Applied Microbiology
Year: 2018
Volume: 41
Issue: 5
Pages: 427-436
Print publication date: 01/09/2018
Online publication date: 04/04/2018
Acceptance date: 22/03/2018
Date deposited: 07/06/2018
ISSN (print): 0723-2020
ISSN (electronic): 1618-0984
Publisher: Elsevier GmbH
URL: https://doi.org/10.1016/j.syapm.2018.03.005
DOI: 10.1016/j.syapm.2018.03.005
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