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Genipin-crosslinked Chitosan Hydrogels as Scaffolds for Mammalian Cell Growth

Lookup NU author(s): Dr Mark Birch, Dr Katarina Novakovic


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While hydrogels can take many forms, covalently crosslinked structures are favoured when enhanced stability andincreased mechanical properties are required. One element of particular relevance to covalently crosslinked hydrogelstructures is the crosslinking agent involved in the scaffold formation, where at present, non-toxic options are limited. Recentadvances in research suggest that genipin is a beneficial crosslinking agent to consider. When such hydrogels are intended asscaffolds for mammalian cells in tissue engineering applications, further consideration must be given to biocompatibility,porosity and pore interconnection, so that appropriate cell activity within the bulk of the scaffold is achieved. Therefore, in thiswork, the viability of genipin-crosslinked chitosan-polyvinylpyrrolidone hydrogels for culturing non-differentiated adultmesenchymal stem cells is studied. Cell viability was observed through fluorescent microscopy and cell number assessed usingthe MTT assay. Results confirmed that these hydrogels are benign to studied cells. Furthermore, it was shown that cellproliferation propagates beneath the gel surface via interconnected pores within the hydrogels.

Publication metadata

Author(s): Chung KY, Birch MA, Novakovic K

Publication type: Article

Publication status: Published

Journal: International Journal of Advances in Science Engineering and Technology

Year: 2018

Volume: 6

Issue: 1

Pages: 37-44

Print publication date: 01/01/2018

Online publication date: 01/01/2018

Acceptance date: 29/11/2017

ISSN (print): 2321-8991

ISSN (electronic): 2321-9009

Publisher: IRAJ Publisher




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