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Characterization of the first cultured representative of a Bacteroidetes clade specialized on the scavenging of cyanobacteria

Lookup NU author(s): Emeritus Professor Hans-Peter Klenk

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).

Abstract

© 2016 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd The anaerobic, mesophilic and moderately halophilic strain L21-Spi-D4T was recently isolated from the suboxic zone of a hypersaline cyanobacterial mat using protein-rich extracts of Arthrospira (formerly Spirulina) platensis as substrate. Phylogenetic analyses based on 16S rRNA genes indicated an affiliation of the novel strain with the Bacteroidetes clade MgMjR-022, which is widely distributed and abundant in hypersaline microbial mats and heretofore comprised only sequences of uncultured bacteria. Analyses of the complete genome sequence of strain L21-Spi-D4T revealed a possible specialization on the degradation of cyanobacterial biomass. Besides genes for enzymes degrading specific cyanobacterial proteins a conspicuous transport complex for the polypeptide cyanophycin could be identified that is homologous to typical polysaccharide utilization loci of Bacteroidetes. A distinct and reproducible co-occurrence pattern of environmental 16S rRNA gene sequences of the MgMjR-022 clade and cyanobacteria in the suboxic zone of hypersaline mats points to a specific dependence of members of this clade on decaying cyanobacteria. Based on a comparative analysis of phenotypic, genomic and ecological characteristics we propose to establish the novel taxa Salinivirga cyanobacteriivorans gen. nov., sp. nov., represented by the type strain L21-Spi-D4T, and Salinivirgaceae fam. nov., comprising sequences of the MgMjR-022 clade.

Publication metadata

Author(s): Ben Hania W, Joseph M, Bunk B, Sproer C, Klenk H-P, Fardeau M-L, Spring S

Publication type: Article

Publication status: Published

Journal: Environmental Microbiology

Year: 2017

Volume: 19

Issue: 3

Pages: 1134-1148

Print publication date: 01/03/2017

Online publication date: 10/12/2016

Acceptance date: 01/12/2016

Date deposited: 09/08/2018

ISSN (print): 1462-2912

ISSN (electronic): 1462-2920

Publisher: Wiley-Blackwell

URL: https://doi.org/10.1111/1462-2920.13639

DOI: 10.1111/1462-2920.13639

PubMed id: 27943642

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