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Lookup NU author(s): Emeritus Professor Hans-Peter Klenk
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© 2017 Elsevier Masson SAS An actinobacterial strain, HG29, with potent activity against pathogenic, toxigenic and phytopathogenic fungi was isolated from a Saharan soil sample of Algeria. On the basis of morphological and chemotaxonomic characteristics, the strain was classified in the genus Streptomyces. Analysis of the 16S rRNA gene sequence showed a similarity level of 99.3% with Streptomyces gancidicus NBRC 15412T. The comparison of its cultural and physiological characteristics with this species revealed significant differences. Moreover, the phylogenetic tree showed that strain HG29 forms a distinct phyletic line within the genus Streptomyces. Production of antifungal activity was investigated by following kinetics in shake broth. The highest antifungal activity was obtained after five days of fermentation, and in the dichloromethane extract. Two active compounds, NK1 and NK2, were purified by HPLC using a C18 column. Their chemical structures were identified through nuclear magnetic resonance experiments and mass spectrometry as oligomycins E and A, respectively, which have not been reported to be produced by S. gancidicus. The two bioactive compounds exhibited significant antifungal activity in vitro, showing minimal inhibitory concentrations (MICs) values between 2 and 75 μg/mL.
Author(s): Khebizi N, Boudjella H, Bijani C, Bouras N, Klenk H-P, Pont F, Mathieu F, Sabaou N
Publication type: Article
Publication status: Published
Journal: Journal de Mycologie Médicale
Year: 2018
Volume: 28
Issue: 1
Pages: 150-160
Print publication date: 01/03/2018
Online publication date: 20/11/2017
Acceptance date: 23/10/2017
ISSN (print): 1156-5233
ISSN (electronic): 1773-0449
Publisher: Elsevier Masson SAS
URL: https://doi.org/10.1016/j.mycmed.2017.10.007
DOI: 10.1016/j.mycmed.2017.10.007
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