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Streptomyces odonnellii sp. nov., a proteolytic streptomycete isolated from soil under cerrado (savanna) vegetation cover

Lookup NU author(s): Emeritus Professor Hans-Peter Klenk

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Abstract

© 2017 IUMS. A novel streptomycete, strain 594T, isolated from Brazilian soil collected under cerrado (savanna) vegetation cover is described. Strain 594T produced thermophilic chitinolytic proteases in assays containing feather meal and corn steep liquor as sole sources of carbon and nitrogen. The strain produced white to grey aerial mycelium and spiral chains of spinysurfaced spores on the aerial mycelium and did not produce diffusible pigments. The LL-isomer of diaminopimelic acid was present in the cell wall and menaquinones were predominantly MK-9(H6) (52 %) and MK-9(H8) (30 %) with 6% MK-9(H4) and slightly less than 1% MK-9(H2). Polar lipids present were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown phospholipid. The major fatty acids were anteiso-C15: 0, anteiso- C16: 0, anteiso-C14: 0 and anteiso-C17: 0. The G+C content of the genomic DNA was 70.4 mol%. Phylogenetic analysis of the nearly complete 16S rRNA gene sequence indicated that it differed from described Streptomyces species. Multilocus sequence analysis (MLSA) using five housekeeping genes (atpD, gyrB, rpoB, recA and trpB) comparing Streptomyces type strains showed that the MLSA distance of strain 594T to the most closely related species was greater than the 0.007 threshold. The in silico DNA–DNA relatedness between the genome sequence of strain 594T and that of the phylogenetically nearest species was well below the species level recommendation. There was thus multiple evidence justifying the description of this strain as representing a novel species, for which the name Streptomyces odonnellii sp. nov. is proposed. The type strain is 594T (=IMPPG 594T=DSM 41949T=NRRL B-24891T).

Publication metadata

Author(s): Pereira PHF, Macrae A, Reinert F, De Souza RF, Coelho RRR, Potter G, Klenk H-P, Labeda DP

Publication type: Article

Publication status: Published

Journal: International Journal of Systematic and Evolutionary Microbiology

Year: 2017

Volume: 67

Issue: 12

Pages: 5211-5215

Print publication date: 01/12/2017

Acceptance date: 12/10/2017

ISSN (print): 1466-5026

ISSN (electronic): 1466-5034

Publisher: Microbiology Society

URL: https://doi.org/10.1099/ijsem.0.002446

DOI: 10.1099/ijsem.0.002446

PubMed id: 29087276

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