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Lookup NU author(s): Dr Piergiorgio GentileORCiD,
Dr Ana Ferreira-DuarteORCiD
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
Hypothesis: Wound healing is a complex process that often requires treatment with antibacterial agents to avoidinfection, which affects the optimal tissue regeneration process. Ideal scaffolds for wound healing treatmentshould combine biomimetic features to ensure the tissue growth on properly designed extracellular matrix(ECM)-like scaffolds and antibacterial properties in order to avoid bacterial colonization.Experiments: In this work, gelatin cross-linked nanofibers (GL-nanofibres), with diameters ranging from 200 to300 nm, were prepared via a “green electrospinning technique” to mimic the structure and composition of theextracellular matrix (ECM), and promote the normal skin wound healing process. Nanofibres were doped withtwo antibacterial agents (gentamicin sulphate or silver nanoparticles) to achieve an antibacterial effect againstGram+and Gram- bacteria.Findings: The ECM-mimicking structure of GL-nanofibres was not affected by the presence of the antibacterialagents, which were homogeneously distributed within the mats as shown by SEM and EDS. The antibacterialproperties of the developed matrices were confirmed using 4 strains (S. aureus, S. epidermidis, P. aeruginosa and E.coli) while the biocompatibility of the developed substrates and their ability to induce cell growth was assessedusing Neonatal Normal Human Dermal Fibroblasts (NHDF-Neo).
Author(s): Tonda-Turo C, Ruini F, Ceresa C, Gentile P, Varela P, Ferreira AM, Fracchia L, Ciardelli G
Publication type: Article
Publication status: Published
Journal: Colloids and Surfaces B: Biointerfaces
Print publication date: 01/12/2018
Online publication date: 18/08/2018
Acceptance date: 17/08/2018
Date deposited: 18/10/2018
ISSN (print): 0927-7765
ISSN (electronic): 1873-4367
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