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Nanostructured scaffold with biomimetic and antibacterial properties for wound healing produced by 'green electrospinning'

Lookup NU author(s): Dr Piergiorgio Gentile, Dr Ana Ferreira-Duarte

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).


Abstract

Hypothesis: Wound healing is a complex process that often requires treatment with antibacterial agents to avoidinfection, which affects the optimal tissue regeneration process. Ideal scaffolds for wound healing treatmentshould combine biomimetic features to ensure the tissue growth on properly designed extracellular matrix(ECM)-like scaffolds and antibacterial properties in order to avoid bacterial colonization.Experiments: In this work, gelatin cross-linked nanofibers (GL-nanofibres), with diameters ranging from 200 to300 nm, were prepared via a “green electrospinning technique” to mimic the structure and composition of theextracellular matrix (ECM), and promote the normal skin wound healing process. Nanofibres were doped withtwo antibacterial agents (gentamicin sulphate or silver nanoparticles) to achieve an antibacterial effect againstGram+and Gram- bacteria.Findings: The ECM-mimicking structure of GL-nanofibres was not affected by the presence of the antibacterialagents, which were homogeneously distributed within the mats as shown by SEM and EDS. The antibacterialproperties of the developed matrices were confirmed using 4 strains (S. aureus, S. epidermidis, P. aeruginosa and E.coli) while the biocompatibility of the developed substrates and their ability to induce cell growth was assessedusing Neonatal Normal Human Dermal Fibroblasts (NHDF-Neo).


Publication metadata

Author(s): Tonda-Turo C, Ruini F, Ceresa C, Gentile P, Varela P, Ferreira AM, Fracchia L, Ciardelli G

Publication type: Article

Publication status: Published

Journal: Colloids and Surfaces B: Biointerfaces

Year: 2018

Volume: 172

Pages: 233-243

Print publication date: 01/12/2018

Online publication date: 18/08/2018

Acceptance date: 17/08/2018

ISSN (print): 0927-7765

ISSN (electronic): 1873-4367

Publisher: Elsevier

URL: https://doi.org/10.1016/j.colsurfb.2018.08.039

DOI: 10.1016/j.colsurfb.2018.08.039


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