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Lookup NU author(s): Dr Seamus Holden
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
Rod-shaped bacteria typically grow first via sporadic and dispersed elongation along their lateral walls and then via a combination of zonal elongation and constriction at the division site to form the poles of daughter cells. Although constriction comprises up to half of the cell cycle, its impact on cell size control and homeostasis has rarely been considered. To reveal the roles of cell elongation and constriction in bacterial size regulation during cell division, we captured the shape dynamics of Caulobacter crescentus with time-lapse structured illumination microscopy and used molecular markers as cell-cycle landmarks. We perturbed the constriction rate using a hyperconstriction mutant or fosfomycin ([(2R,3S)-3-methyloxiran-2-yl]phosphonic acid) inhibition. We report that the constriction rate contributes to both size control and homeostasis, by determining elongation during constriction and by compensating for variation in pre-constriction elongation on a single-cell basis.
Author(s): Lambert A, Vanhecke A, Archetti A, Holden S, Schaber F, Pincus Z, Laub MT, Goley E, Manley S
Publication type: Article
Publication status: Published
Journal: iScience
Year: 2018
Volume: 4
Pages: 180-189
Print publication date: 29/06/2018
Online publication date: 30/05/2018
Acceptance date: 24/05/2018
Date deposited: 18/09/2018
ISSN (electronic): 2589-0042
Publisher: Cell Press
URL: https://doi.org/10.1016/j.isci.2018.05.020
DOI: 10.1016/j.isci.2018.05.020
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