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The synthesis of novel chromogenic enzyme substrates for detection of bacterial glycosidases and their applications in diagnostic microbiology

Lookup NU author(s): Dr John Perry

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Abstract

© 2018 Elsevier Ltd The preparation and evaluation of chromogenic substrates for detecting bacterial glycosidase enzymes is reported. These substrates are monoglycoside derivatives of the metal chelators catechol, 2,3-dihydroxynaphthalene (DHN) and 6,7-dibromo-2,3-dihydroxynaphthalene (6,7-dibromo-DHN). When hydrolysed by appropriate bacterial enzymes these substrates produced coloured chelates in the presence of ammonium iron(III) citrate, thus enabling bacterial detection. A β-D-riboside of DHN and a β-D-glucuronide derivative of 6,7-dibromo-DHN were particularly effective for the detection of S. aureus and E. coli respectively.

Publication metadata

Author(s): Burton M, Perry JD, Stanforth SP, Turner HJ

Publication type: Article

Publication status: Published

Journal: Bioorganic and Medicinal Chemistry

Year: 2018

Volume: 26

Issue: 17

Pages: 4841-4849

Print publication date: 15/09/2018

Online publication date: 17/08/2018

Acceptance date: 16/08/2018

ISSN (print): 0968-0896

ISSN (electronic): 1464-3391

Publisher: Elsevier Ltd

URL: https://doi.org/10.1016/j.bmc.2018.08.023

DOI: 10.1016/j.bmc.2018.08.023

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