Lookup NU author(s): Dr Elisa Molinari,
Dr Simon Ramsbottom,
Professor John Sayer
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2018 Molinari E et al. Zebrafish are a valuable vertebrate model in which to study development and characterize genes involved in cystic kidney disease. Zebrafish embryos and larvae are transparent, allowing non-invasive imaging during their rapid development, which takes place over the first 72 hours post fertilisation. Gene-specific knockdown of nephronophthisis-associated genes leads to ciliary phenotypes which can be assessed in various developmental structures. Here we describe in detail the methods used for imaging cilia within Kupffer’s vesicle to assess nephronophthisis and related ciliopathy phenotypes.
Author(s): Molinari E, Ramsbottom SA, Sammut V, Hughes FEP, Sayer JA
Publication type: Article
Publication status: Published
Online publication date: 25/07/2018
Acceptance date: 02/04/2018
Date deposited: 26/09/2018
ISSN (print): 2046-1402
ISSN (electronic): 1759-796X
Publisher: F1000 Research Ltd
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