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Lookup NU author(s): Samet Sahin, James Merotra, Dr Jing Kang, Professor Mike TrenellORCiD, Professor Mike Catt, Professor Eileen Yu
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IEEE In this work, the feasibility of simultaneous electrochemical detection of glucose and non-esterified fatty acids (NEFA) is investigated using enzyme-based biosensors. The detection of glucose is based on ferrocene-Nafion-glucose oxidase (Fc-Nafion-GOx) modified screen-printed electrodes (SPEs). Solutions of Fc-Nafion-GOx were drop casted on SPEs and dried at room temperature allowing immobilisation of GOx entrapped in Nafion polymer with Fc. The detection of NEFA is based on bienzyme electrodes fabricated by the layer by layer (LbL) technique applied onto SPEs using multilayers of poly(dimethyldiallyammonium chloride) polymer, multi-walled carbon nanotube composite films and two enzymes: acyl-CoA synthetase and acyl-CoA oxidase. Oleoyl coenzyme A lithium salt (OACoA) and oleic acid (OA) were used as NEFA of interests because they are two of the most employed markers in clinical tests. Preliminary results demonstrated that enzyme electrodes showed linear response within the physiological concentration range of glucose and OA in human blood plasma as 5 mM and 1.2 mM, respectively. These results indicate a promising multibiosensor platform for rapid one-step determination of glucose and NEFA simultaneously. This could lead the way towards the fabrication of glucose-NEFA biosensors which can be important in diabetes management and early diagnosis.
Author(s): Sahin S, Merotra J, Kang J, Trenell M, Catt M, Yu EH
Publication type: Article
Publication status: Published
Journal: IEEE Sensors Journal
Year: 2018
Volume: 18
Issue: 22
Pages: 9075-9080
Print publication date: 15/11/2018
Online publication date: 17/09/2018
Acceptance date: 02/04/2018
ISSN (print): 1530-437X
ISSN (electronic): 1558-1748
Publisher: IEEE
URL: https://doi.org/10.1109/JSEN.2018.2870071
DOI: 10.1109/JSEN.2018.2870071
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