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Frankia canadensis sp. Nov., isolated from root nodules of Alnus incana subspecies rugosa

Lookup NU author(s): Dr Imen Nouioui, Emeritus Professor Hans-Peter Klenk

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Abstract

© 2018 IUMS. Strain ARgP5T, an actinobacterium isolated from a root nodule present on an Alnus incana subspecies rugosa shrub growing in Quebec City, Canada, was the subject of polyphasic taxonomic studies to clarify its status within the genus Frankia. 16S rRNA gene sequence similarities and ANI values between ARgP5T and type strains of species of the genus Frankia with validly published names were 98.8 and 82% or less, respectively. The in silico DNA G+C content was 72.4 mol%. ARgP5T is characterised by the presence of meso-A2pm, galactose, glucose, mannose, rhamnose (trace), ribose and xylose as wholeorganism hydrolysates; MK-9(H8) as predominant menaquinone; diphosphatidylglycerol, phosphatidylinositol and phosphatidylglycerol as polar lipids and iso-C16: 0 and C17: 1ω8c as major fatty acids. The proteomic results confirmed the distinct position of ARgP5T from its closest neighbours in Frankia cluster 1. ARgP5T was found to be infective on two alder (Alnus glutinosa and Alnusalnobetula subsp. crispa) and on one bayberry (Morella pensylvanica) species and to fix nitrogen in symbiosis and in pure culture. On the basis of phylogenetic (16S rRNA gene sequence), genomic, proteomic and phenotypic results, strain ARgP5T (=DSM 45898=CECT 9033) is considered to represent a novel species within the genus Frankia for which the name Frankia canadensis sp. nov., is proposed.


Publication metadata

Author(s): Normand P, Nouioui I, Pujic P, Fournier P, Dubost A, Schwob G, Klenk H-P, Nguyen A, Abrouk D, Belaroussi AH, Pothier JF, Pfluger V, Fernandez MP

Publication type: Article

Publication status: Published

Journal: International Journal of Systematic and Evolutionary Microbiology

Year: 2018

Volume: 68

Issue: 9

Pages: 3001-3011

Online publication date: 01/09/2018

Acceptance date: 10/07/2018

ISSN (print): 1466-5026

ISSN (electronic): 1466-5034

Publisher: Microbiology Society

DOI: 10.1099/ijsem.0.002939


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