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Lookup NU author(s): Dr Emanuela Torelli
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A prototype for a DNA origami nanorobot has been designed, produced and tested. The 3D cylindrical nanorobot (14x14x48 nm) with a switchable flap, is able to detect picomol amounts of a DNA target and reveal it using a DNAzyme as the biocatalytic label.The actuation switch is triggered by an external nucleic acid (red) that interacts with a complementary nucleic acid (yellow) that is beard externally by the nanostructure.[1,2]Upon actuation the nanorobot moves the flap and a DNAzyme (green) was pulled out of the cylinder and exposed to hemin (gold): the hemin/G-quadruplex horseradish peroxidase mimicking DNAzyme catalyzes a colorimetric reaction or chemiluminescence generation.The nanorobotinternal cavity (3 zeptoliters) is large enough to accomodate a functional cargo, while the nano-object is small enough to be entirely contained within the capsid of viruses.This work opens perspectives for the construction of nanorobots that can be hosted within cellular delivery vectors and can host, concurrently, selectively accessible molecular payloads in their internal cavity.References E. Torelli et al. 2014. A DNA Origami Nanorobot Controlled by Nucleic Acid Hybridization. Small. First published on line.  M. Marini et al. 2011. A Revertible, Autonomous, Self-assembled DNA-Origami Nanoactuator. Nano Lett. 11, 5449-5454.  J. Mikkila et al. 2014. Virus encapsulated DNA origami nanostructures for cellular delivery. Nano Lett. 14: 2196-2200.
Author(s): Torelli E, Marini M, Palmano S, Piantanida L, Polano C, Scarpellini A, Lazzarino M, Firrao G
Editor(s): Ricci, F.
Publication type: Conference Proceedings (inc. Abstract)
Publication status: Published
Conference Name: Functional DNA Nanotechnology
Year of Conference: 2014
Acceptance date: 14/04/2014