Toggle Main Menu Toggle Search

Open Access padlockePrints

Switchable 3D DNA Origami Nanorobot

Lookup NU author(s): Dr Emanuela Torelli


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


A prototype for a DNA origami nanorobot has been designed, produced and tested. The 3D cylindrical nanorobot (14x14x48 nm) with a switchable flap, is able to detect picomol amounts of a DNA target and reveal it using a DNAzyme as the biocatalytic label.The actuation switch is triggered by an external nucleic acid (red) that interacts with a complementary nucleic acid (yellow) that is beard externally by the nanostructure.[1,2]Upon actuation the nanorobot moves the flap and a DNAzyme (green) was pulled out of the cylinder and exposed to hemin (gold): the hemin/G-quadruplex horseradish peroxidase mimicking DNAzyme catalyzes a colorimetric reaction or chemiluminescence generation.[1]The nanorobotinternal cavity (3 zeptoliters) is large enough to accomodate a functional cargo, while the nano-object is small enough to be entirely contained within the capsid of viruses.[3]This work opens perspectives for the construction of nanorobots that can be hosted within cellular delivery vectors and can host, concurrently, selectively accessible molecular payloads in their internal cavity.References[1] E. Torelli et al. 2014. A DNA Origami Nanorobot Controlled by Nucleic Acid Hybridization. Small. First published on line. [2] M. Marini et al. 2011. A Revertible, Autonomous, Self-assembled DNA-Origami Nanoactuator. Nano Lett. 11, 5449-5454. [3] J. Mikkila et al. 2014. Virus encapsulated DNA origami nanostructures for cellular delivery. Nano Lett. 14: 2196-2200.

Publication metadata

Author(s): Torelli E, Marini M, Palmano S, Piantanida L, Polano C, Scarpellini A, Lazzarino M, Firrao G

Editor(s): Ricci, F.

Publication type: Conference Proceedings (inc. Abstract)

Publication status: Published

Conference Name: Functional DNA Nanotechnology

Year of Conference: 2014

Acceptance date: 14/04/2014