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A Comparison of Simple Analytical Methods for Determination of Fluoride in Microlitre-Volume Plasma Samples

Lookup NU author(s): Dr Vida Zohoori, Professor Anne Maguire, Professor Marilia Buzalaf, Dr Roy Sanderson

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This is the authors' accepted manuscript of an article that has been published in its final definitive form by S. Karger AG, 2019.

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Abstract

© 2018 S. Karger AG, Basel. The aim was to compare potential methods for fluoride analysis in microlitre-volume plasma samples containing nano-gram amounts of fluoride. Methods: A group of 4 laboratories analysed a set of standardised biological samples as well as plasma to determine fluoride concentration using 3 methods. In Phase-1, fluoride analysis was carried out using the established hexamethyldisiloxane (HMDS)-diffusion method (1 mL-aliquot/analysis) to obtain preliminary measurement of agreement between the laboratories. In Phase-2, the laboratories analysed the same samples using a micro-diffusion method and known-addition technique with 200 μL-aliquot/analysis. Coefficients of Variation (CVs) and intra-class correlation coefficients (ICCs) were estimated using analysis of variance to evaluate the amount of variation within- and between-laboratories. Based on the results of the Phase-2 analysis, 20 human plasma samples were analysed and compared using the HMDS-diffusion method and known-addition technique in Phase-3. Results: Comparison of Phase-1 results showed no statistically significant difference among the laboratories for the overall data set. The mean between- and within-laboratory CVs and ICCs were < 0.13 and ≥0.99, respectively, indicating very low variability and excellent reliability. In Phase-2, the overall results for between-laboratory variability showed a poor CV (1.16) and ICC (0.44) for the micro-diffusion method, whereas with the known-addition technique the corresponding values were 0.49 and 0.83. Phase-3 results showed no statistically significant difference in fluoride concentrations of the plasma samples measured with HMDS-diffusion method and known- addition technique, with a mean (SE) difference of 0.002 (0.003) μg/mL. In conclusion, the known-addition technique could be a suitable alternative for the measurement of fluoride in plasma with microlitre-volume samples.


Publication metadata

Author(s): Zohoori FV, Maguire A, Martinez-Mier EA, Buzalaf MAR, Sanderson R, Eckert GJ

Publication type: Article

Publication status: Published

Journal: Caries Research

Year: 2019

Volume: 53

Issue: 3

Pages: 275-283

Online publication date: 08/10/2018

Acceptance date: 18/07/2018

Date deposited: 24/11/2018

ISSN (print): 0008-6568

ISSN (electronic): 1421-976X

Publisher: S. Karger AG

URL: https://doi.org/10.1159/000492339

DOI: 10.1159/000492339


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