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Lookup NU author(s): Dr Vida Zohoori,
Emerita Professor Anne Maguire,
Professor Marilia Buzalaf,
Dr Roy Sanderson
This is the authors' accepted manuscript of an article that has been published in its final definitive form by S. Karger AG, 2019.
For re-use rights please refer to the publisher's terms and conditions.
© 2018 S. Karger AG, Basel. The aim was to compare potential methods for fluoride analysis in microlitre-volume plasma samples containing nano-gram amounts of fluoride. Methods: A group of 4 laboratories analysed a set of standardised biological samples as well as plasma to determine fluoride concentration using 3 methods. In Phase-1, fluoride analysis was carried out using the established hexamethyldisiloxane (HMDS)-diffusion method (1 mL-aliquot/analysis) to obtain preliminary measurement of agreement between the laboratories. In Phase-2, the laboratories analysed the same samples using a micro-diffusion method and known-addition technique with 200 μL-aliquot/analysis. Coefficients of Variation (CVs) and intra-class correlation coefficients (ICCs) were estimated using analysis of variance to evaluate the amount of variation within- and between-laboratories. Based on the results of the Phase-2 analysis, 20 human plasma samples were analysed and compared using the HMDS-diffusion method and known-addition technique in Phase-3. Results: Comparison of Phase-1 results showed no statistically significant difference among the laboratories for the overall data set. The mean between- and within-laboratory CVs and ICCs were < 0.13 and ≥0.99, respectively, indicating very low variability and excellent reliability. In Phase-2, the overall results for between-laboratory variability showed a poor CV (1.16) and ICC (0.44) for the micro-diffusion method, whereas with the known-addition technique the corresponding values were 0.49 and 0.83. Phase-3 results showed no statistically significant difference in fluoride concentrations of the plasma samples measured with HMDS-diffusion method and known- addition technique, with a mean (SE) difference of 0.002 (0.003) μg/mL. In conclusion, the known-addition technique could be a suitable alternative for the measurement of fluoride in plasma with microlitre-volume samples.
Author(s): Zohoori FV, Maguire A, Martinez-Mier EA, Buzalaf MAR, Sanderson R, Eckert GJ
Publication type: Article
Publication status: Published
Journal: Caries Research
Print publication date: 01/04/2019
Online publication date: 08/10/2018
Acceptance date: 18/07/2018
Date deposited: 24/11/2018
ISSN (print): 0008-6568
ISSN (electronic): 1421-976X
Publisher: S. Karger AG
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