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Lookup NU author(s): Professor Nicholas JakubovicsORCiD
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There is increasing recognition of the emerging role of manganese regulation and acquisition in some pathogenic bacteria. Expression of the Enterococcus faecalis endocarditis-associated virulence factor EfaA is induced by growth in serum. It is demonstrated here that expression of the efaCBA operon encoding a putative ABC-type transporter is regulated by Mn(2+). Transcription of efaCBA and EfaA production were repressed in Mn(2+)-supplemented medium. A Mn(2+)-responsive transcriptional regulator, EfaR, sharing 27 % identity with the Corynebacterium diphtheriae diphtheria toxin repressor (DtxR), was identified. In the presence of Mn(2+), EfaR protein bound in vitro to the efaC promoter region. Analysis of the E. faecalis V583 genome revealed ten additional putative EfaR-binding sites, suggesting that manganese availability could have a broader regulatory role in infection. The results identify a new Mn(2+)-sensing regulator in enterococci that regulates the expression of a virulence factor implicated in enterococcal endocarditis.
Author(s): Jakubovics NS; Low YL; Flatman JC; Jenkinson HF; Smith AW
Publication type: Article
Publication status: Published
Journal: Journal of Medical Microbiology
ISSN (print): 0022-2615
ISSN (electronic): 1473-5644
Publisher: Society for General Microbiology
Notes: Journal Article
Research Support, Non-U.S. Gov't
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