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Newly-designed collagen/polyurethane bioartificial blend as coating on bioactive glass-ceramics for bone tissue engineering applications

Lookup NU author(s): Dr Piergiorgio GentileORCiD



This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).


© 2018 In the present work, a new combination of synthetic and natural biomaterials is proposed for bone tissue engineering (BTE) applications. In order to mimic the inorganic and organic phases of bone extracellular matrix (ECM), a bioactive glass-ceramic deriving from a SiO2–P2O5–CaO–MgO–Na2O–K2O parent glass, acting as a substrate in form of a slice, was surface-functionalised with a type I collagen-based coating. In particular, the collagen was blended with a water soluble polyurethane (PUR), synthesised from poly(ethylene glycol), 1,6-hexamethylene diisocyanate and N-BOC-serinol. The PUR was designed to expose amino groups on the polymeric chain, which can be exploited for the blend stabilisation through crosslinking. The newly synthesised PUR demonstrated to be non-cytotoxic, as assessed by a biological test with MG-63 osteoblast-like cells. The collagen/PUR blend showed good biocompatibility as well. The polymeric coating on the glass-ceramic samples was produced by surface-silanisation, followed by further chemical grafting of the blend, using genipin as a crosslinker. The glass-ceramic surface was characterised at each functionalisation step, demonstrating that the procedure allowed obtaining a covalent link between the blend and the substrate. Finally, biological tests performed using human periosteal derived precursor cells demonstrated that the proposed polymer-coated material was a good substrate for bone cell adhesion and growth, and a good candidate to mimic the composite nature of the bone ECM.

Publication metadata

Author(s): Caddeo S, Mattioli-Belmonte M, Cassino C, Barbani N, Dicarlo M, Gentile P, Baino F, Sartori S, Vitale-Brovarone C, Ciardelli G

Publication type: Article

Publication status: Published

Journal: Materials Science and Engineering C

Year: 2019

Volume: 96

Pages: 218-233

Online publication date: 10/11/2018

Acceptance date: 07/11/2018

Date deposited: 07/12/2018

ISSN (print): 0928-4931

ISSN (electronic): 1873-0191

Publisher: Elsevier Ltd


DOI: 10.1016/j.msec.2018.11.012


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