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Lookup NU author(s): Dr Kamilla Mahkamova, Dr Nani Md Latar, Sebastian Aspinall, Dr Annette Meeson
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
Comparison of studies of cells derived from normal and pathological tissues of the same organ can be fraught with difficulties, particular with cancer where a number of different diseases are considered cancer within the same tissue. In the thyroid, there are 4 main types of cancer, three of which arise from follicular epithelial cells; papillary and follicular which are classified as differentiated, and anaplastic which is classified as undifferentiated. One assay that can be utilised for isolation of cancer stem cells is the side population (SP) assay. However, SP studies have been limited in part due to lack of optimal isolation strategies and in the case of anaplastic thyroid cancer (ATC) are further compounded by lack of access to ATC tumors. We have used thyroid cell lines to determine the optimal conditions to isolate viable SP cells.We then compared SP cells and NSP cells (bulk tumour cells without the SP) of a normal thyroid cell line N-thy ori-3-1 and an anaplastic thyroid cancer cell line SW1736 and showed that both SP cell populations displayed higher levels of stem cell characteristics than the NSP. When we compared SP cells of the N-thy ori-3-1 and the SW1736, the SW1736 SP had a higher colony forming potential, expressed higher levels of stem cell markers and CXCR4 and where more migratory and invasive, invasiveness increasing in response to CXCL12.This is the first report showing functional differences between ATC SP and normal thyroid SP and could lead to the identification of new therapeutic targets to treat ATC.
Author(s): Mahkamova K, Md Latar N, Aspinall S, Meeson A
Publication type: Article
Publication status: Published
Journal: Experimental Cell Research
Year: 2019
Volume: 374
Issue: 1
Pages: 104-113
Print publication date: 01/01/2019
Online publication date: 19/11/2018
Acceptance date: 16/11/2018
Date deposited: 05/12/2018
ISSN (print): 0014-4827
ISSN (electronic): 1090-2422
Publisher: Elsevier
URL: https://doi.org/10.1016/j.yexcr.2018.11.012
DOI: 10.1016/j.yexcr.2018.11.012
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