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Bacterial communities involved directly or indirectly in the anaerobic degradation of cellulose

Lookup NU author(s): Dr Jan DolfingORCiD

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This is the authors' accepted manuscript of an article that has been published in its final definitive form by Springer, 2019.

For re-use rights please refer to the publisher's terms and conditions.


Abstract

© 2019, Springer-Verlag GmbH Germany, part of Springer Nature. To determine bacterial communities involved, directly or indirectly, in the anaerobic degradation of cellulose, we conducted a microcosm experiment with soil treated with 13C-cellulose, 12C-cellulose, or without cellulose with analyses of DNA-based stable isotope probing (DNA-SIP), real-time quantitative PCR, and high-throughput sequencing. Firmicutes, Actinobacteria, Verrucomicrobia, and Fibrobacteres were the dominant bacterial phyla-degrading cellulose. Generally, bacteria possessing gene-encoding enzymes involved in the degradation of cellulose and hemicellulose were stimulated. Phylotypes affiliated to Geobacter were also stimulated by cellulose, probably due to their role in electron transfer. Nitrogen-fixing bacteria were also detected, probably due to the decreased N availability during cellulose degradation. High-throughput sequencing showed the presence of bacteria not incorporating 13C and probably involved in the priming effect caused by the addition of cellulose to soil. Collectively, our findings revealed that a more diverse microbial community than expected directly and indirectly participated in anaerobic cellulose degradation.


Publication metadata

Author(s): Bao Y, Dolfing J, Wang B, Chen R, Huang M, Li Z, Lin X, Feng Y

Publication type: Article

Publication status: Published

Journal: Biology and Fertility of Soils

Year: 2019

Volume: 55

Issue: 3

Pages: 201-211

Print publication date: 01/04/2019

Online publication date: 18/01/2019

Acceptance date: 10/01/2019

Date deposited: 20/03/2019

ISSN (print): 0178-2762

ISSN (electronic): 1432-0789

Publisher: Springer

URL: https://doi.org/10.1007/s00374-019-01342-1

DOI: 10.1007/s00374-019-01342-1


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Funding

Funder referenceFunder name
41471208
2016YFD0200306
41771294
41430859
41671267
ISSASIP1639
SCHM-2018
XDB15020103

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