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Lookup NU author(s): Dr Jan DolfingORCiD
This is the authors' accepted manuscript of an article that has been published in its final definitive form by Springer, 2019.
For re-use rights please refer to the publisher's terms and conditions.
© 2019, Springer-Verlag GmbH Germany, part of Springer Nature. To determine bacterial communities involved, directly or indirectly, in the anaerobic degradation of cellulose, we conducted a microcosm experiment with soil treated with 13C-cellulose, 12C-cellulose, or without cellulose with analyses of DNA-based stable isotope probing (DNA-SIP), real-time quantitative PCR, and high-throughput sequencing. Firmicutes, Actinobacteria, Verrucomicrobia, and Fibrobacteres were the dominant bacterial phyla-degrading cellulose. Generally, bacteria possessing gene-encoding enzymes involved in the degradation of cellulose and hemicellulose were stimulated. Phylotypes affiliated to Geobacter were also stimulated by cellulose, probably due to their role in electron transfer. Nitrogen-fixing bacteria were also detected, probably due to the decreased N availability during cellulose degradation. High-throughput sequencing showed the presence of bacteria not incorporating 13C and probably involved in the priming effect caused by the addition of cellulose to soil. Collectively, our findings revealed that a more diverse microbial community than expected directly and indirectly participated in anaerobic cellulose degradation.
Author(s): Bao Y, Dolfing J, Wang B, Chen R, Huang M, Li Z, Lin X, Feng Y
Publication type: Article
Publication status: Published
Journal: Biology and Fertility of Soils
Year: 2019
Volume: 55
Issue: 3
Pages: 201-211
Print publication date: 01/04/2019
Online publication date: 18/01/2019
Acceptance date: 10/01/2019
Date deposited: 20/03/2019
ISSN (print): 0178-2762
ISSN (electronic): 1432-0789
Publisher: Springer
URL: https://doi.org/10.1007/s00374-019-01342-1
DOI: 10.1007/s00374-019-01342-1
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