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Lookup NU author(s): Dr Seamus Holden
This is the authors' accepted manuscript of an article that has been published in its final definitive form by Nature Publishing Group, 2019.
For re-use rights please refer to the publisher's terms and conditions.
With the widespread uptake of 2D and 3D single molecule localization microscopy, a large set of different data analysis packages have been developed to generate super-resolution images. To guide researchers on the optimal analytical software for their experiments, in a large community effort we designed a competition to extensively characterise and rank these options. We generated realistic simulated datasets for popular imaging modalities – 2D, astigmatic 3D, biplane 3D, and double helix 3D – and evaluated 36 participant packages against these data. This provides the first broad assessment of 3D single molecule localization microscopy software, provides a holistic view of how the latest 2D and 3D single molecule localization software perform in realistic conditions, and ultimately provides insight into the current limits of the field.
Author(s): Sage D, Pham TA, Babcock H, Lukes T, Pengo T, Chao J, Velmuruga R, Herbert A, Agrawal A, Colabrese S, Wheeler A, Archetti A, Rieger B, Ober R, Hagen GM, Sibarita JB, Ries J, Henriques R, Unser M, Holden S
Publication type: Article
Publication status: Published
Journal: Nature Methods
Year: 2019
Volume: 16
Pages: 387-395
Online publication date: 08/04/2019
Acceptance date: 26/02/2019
Date deposited: 06/02/2019
ISSN (print): 1548-7091
ISSN (electronic): 1548-7105
Publisher: Nature Publishing Group
URL: https://doi.org/10.1038/s41592-019-0364-4
DOI: 10.1038/s41592-019-0364-4
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