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Lookup NU author(s): Professor Tracy Palmer FRS FRSE FMedSciORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2016 Federation of European Biochemical Societies.The Ess/Type VII protein secretion system, essential for virulence of pathogenic Staphylococcus aureus, is dependent upon the four core membrane proteins EssA, EssB, EssC and EsaA. Here, we use crosslinking and blue native PAGE analysis to show that the EssB, EssC and EsaA proteins individually form homomeric complexes. Surprisingly, these components appear unable to interact with each other, or with the EssA protein. We further show that two high molecular weight multimers of EssC detected in whole cells are not dependent upon the presence of EsxA, EsxB or any other Ess component for their assembly.
Author(s): Jager F, Zoltner M, Kneuper H, Hunter WN, Palmer T
Publication type: Article
Publication status: Published
Journal: FEBS Letters
Year: 2016
Volume: 590
Issue: 3
Pages: 349-357
Print publication date: 12/02/2016
Online publication date: 20/01/2016
Acceptance date: 12/01/2016
Date deposited: 14/02/2019
ISSN (print): 0014-5793
ISSN (electronic): 1873-3468
Publisher: Wiley Blackwell
URL: https://doi.org/10.1002/1873-3468.12065
DOI: 10.1002/1873-3468.12065
PubMed id: 26785823
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