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Membrane interactions and self-association of components of the Ess/Type VII secretion system of Staphylococcus aureus

Lookup NU author(s): Professor Tracy Palmer FRS FRSE FMedSciORCiD

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

© 2016 Federation of European Biochemical Societies.The Ess/Type VII protein secretion system, essential for virulence of pathogenic Staphylococcus aureus, is dependent upon the four core membrane proteins EssA, EssB, EssC and EsaA. Here, we use crosslinking and blue native PAGE analysis to show that the EssB, EssC and EsaA proteins individually form homomeric complexes. Surprisingly, these components appear unable to interact with each other, or with the EssA protein. We further show that two high molecular weight multimers of EssC detected in whole cells are not dependent upon the presence of EsxA, EsxB or any other Ess component for their assembly.


Publication metadata

Author(s): Jager F, Zoltner M, Kneuper H, Hunter WN, Palmer T

Publication type: Article

Publication status: Published

Journal: FEBS Letters

Year: 2016

Volume: 590

Issue: 3

Pages: 349-357

Print publication date: 12/02/2016

Online publication date: 20/01/2016

Acceptance date: 12/01/2016

Date deposited: 14/02/2019

ISSN (print): 0014-5793

ISSN (electronic): 1873-3468

Publisher: Wiley Blackwell

URL: https://doi.org/10.1002/1873-3468.12065

DOI: 10.1002/1873-3468.12065

PubMed id: 26785823


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Funding

Funder referenceFunder name
TP is a Royal Society/Wolfson merit award holder
UK Biotechnology and Biological Sciences Research Council EASTBIO Doctoral Training Partnership. Grant Number: BB/J01446X/1
UK Medical Research Council. Grant Number: MR/M011224/1

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