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Lookup NU author(s): Professor William WillatsORCiD
This is the authors' accepted manuscript of an article that has been published in its final definitive form by Springer Verlag, 2019.
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© 2019, Springer-Verlag GmbH Germany, part of Springer Nature. Main conclusion: Evidence is presented that cotton fibre adhesion and middle lamella formation are preceded by cutin dilution and accompanied by rhamnogalacturonan-I metabolism. Cotton fibres are single cell structures that early in development adhere to one another via the cotton fibre middle lamella (CFML) to form a tissue-like structure. The CFML is disassembled around the time of initial secondary wall deposition, leading to fibre detachment. Observations of CFML in the light microscope have suggested that the development of the middle lamella is accompanied by substantial cell-wall metabolism, but it has remained an open question as to which processes mediate adherence and which lead to detachment. The mechanism of adherence and detachment were investigated here using glyco-microarrays probed with monoclonal antibodies, transcript profiling, and observations of fibre auto-digestion. The results suggest that adherence is brought about by cutin dilution, while the presence of relevant enzyme activities and the dynamics of rhamnogalacturonan-I side-chain accumulation and disappearance suggest that both attachment and detachment are accompanied by rhamnogalacturonan-I metabolism.
Author(s): Guo X, Runavot J-L, Bourot S, Meulewaeter F, Hernandez-Gomez M, Holland C, Harholt J, Willats WGT, Mravec J, Knox P, Ulvskov P
Publication type: Article
Publication status: Published
Journal: Planta
Year: 2019
Volume: 249
Pages: 1565-1581
Online publication date: 08/02/2019
Acceptance date: 04/02/2019
Date deposited: 09/04/2019
ISSN (print): 0032-0935
ISSN (electronic): 1432-2048
Publisher: Springer Verlag
URL: https://doi.org/10.1007/s00425-019-03107-4
DOI: 10.1007/s00425-019-03107-4
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