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Metabolism of polysaccharides in dynamic middle lamellae during cotton fibre development

Lookup NU author(s): Professor William WillatsORCiD



This is the authors' accepted manuscript of an article that has been published in its final definitive form by Springer Verlag, 2019.

For re-use rights please refer to the publisher's terms and conditions.


© 2019, Springer-Verlag GmbH Germany, part of Springer Nature. Main conclusion: Evidence is presented that cotton fibre adhesion and middle lamella formation are preceded by cutin dilution and accompanied by rhamnogalacturonan-I metabolism. Cotton fibres are single cell structures that early in development adhere to one another via the cotton fibre middle lamella (CFML) to form a tissue-like structure. The CFML is disassembled around the time of initial secondary wall deposition, leading to fibre detachment. Observations of CFML in the light microscope have suggested that the development of the middle lamella is accompanied by substantial cell-wall metabolism, but it has remained an open question as to which processes mediate adherence and which lead to detachment. The mechanism of adherence and detachment were investigated here using glyco-microarrays probed with monoclonal antibodies, transcript profiling, and observations of fibre auto-digestion. The results suggest that adherence is brought about by cutin dilution, while the presence of relevant enzyme activities and the dynamics of rhamnogalacturonan-I side-chain accumulation and disappearance suggest that both attachment and detachment are accompanied by rhamnogalacturonan-I metabolism.

Publication metadata

Author(s): Guo X, Runavot J-L, Bourot S, Meulewaeter F, Hernandez-Gomez M, Holland C, Harholt J, Willats WGT, Mravec J, Knox P, Ulvskov P

Publication type: Article

Publication status: Published

Journal: Planta

Year: 2019

Volume: 249

Pages: 1565-1581

Online publication date: 08/02/2019

Acceptance date: 04/02/2019

Date deposited: 09/04/2019

ISSN (print): 0032-0935

ISSN (electronic): 1432-2048

Publisher: Springer Verlag


DOI: 10.1007/s00425-019-03107-4


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