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Lookup NU author(s): Emerita Professor Anne Borland
This is the authors' accepted manuscript of an article that has been published in its final definitive form by Oxford University Press, 2019.
For re-use rights please refer to the publisher's terms and conditions.
Crassulacean acid metabolism (CAM) is a carbon-concentrating mechanism that has evolved numerous times across flowering plants and is thought to be an adaptation to water limited environments. CAM has been investigated from physiological and biochemical perspectives, but little is known about how plants evolve from C3 to CAM at the genetic or metabolic level. Here we take a comparative approach in analyzing time-course data of C3, CAM, and C3+CAM intermediate Yucca (Asparagaceae) species. RNA samples were collected over a 24-hour period from both well-watered and drought-stressed plants and were clustered based on time-dependent expression patterns. Metabolomic data reveals differences in carbohydrate metabolism and antioxidant response between the CAM and C3 species, suggesting changes to metabolic pathways are important for CAM evolution and function. However, all three species share expression profiles of canonical CAM pathway genes, regardless of photosynthetic pathway. Despite differences in transcript and metabolite profiles between the C3 and CAM species, shared time-structured expression of CAM genes in both CAM and C3Yucca species suggests ancestral expression patterns required for CAM may have predated its origin in Yucca.
Author(s): Heyduk K, Ray JN, Ayyampalayam S, Moledina N, Borland A, Harding SA, Tsai C-J, Leebens-Mack J
Publication type: Article
Publication status: Published
Journal: Journal of Experimental Botany
Year: 2019
Volume: 70
Issue: 22
Pages: 6597-6609
Print publication date: 15/11/2019
Online publication date: 13/04/2019
Acceptance date: 20/02/2019
Date deposited: 06/03/2019
ISSN (print): 0022-0957
ISSN (electronic): 1460-2431
Publisher: Oxford University Press
URL: https://doi.org/10.1093/jxb/erz105
DOI: 10.1093/jxb/erz105
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