Browse by author
Lookup NU author(s): Professor Moein MoghimiORCiD
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Cellular toxicity and/or cell death entail complex mechanisms that require multifaceted characterization. A detailed mechanistic assessment of cytotoxicity is essential for design and construction of more effective polycations for nucleic acid delivery. A single toxicity assay cannot stand alone in determining the type and extent of damage or cell death mechanism. In this chapter we describe a lactate dehydrogenase (LDH) assay for high-throughput screening that can be used as a starting point for further detailed cytotoxicity determination. LDH release is considered an early event in necrosis but a late event in apoptosis. An accurate temporal assessment of the toxic responses is crucial as late apoptosis may convert into necrosis as well as in situations where cell death is initiated without any visible cell morphological changes or responses in assays measuring late events, resulting in early ongoing toxicity being overlooked.
Author(s): Parhamifar L, Andersen H, Moghimi SM
Publication type: Book Chapter
Publication status: Published
Book Title: Nanotechnology for Nucleic Acid Delivery: Methods and Protocols
Year: 2019
Volume: 1943
Pages: 291-299
Online publication date: 06/03/2019
Acceptance date: 02/04/2018
Series Title: Methods in Molecular Biology
Publisher: Humana Press
Place Published: New York
URL: https://doi.org/10.1007/978-1-4939-9092-4_18
DOI: 10.1007/978-1-4939-9092-4_18
PubMed id: 30838623
Library holdings: Search Newcastle University Library for this item
ISBN: 9781493990917