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Lactate Dehydrogenase Assay for Assessment of Polycation Cytotoxicity

Lookup NU author(s): Professor Moein MoghimiORCiD


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Cellular toxicity and/or cell death entail complex mechanisms that require multifaceted characterization. A detailed mechanistic assessment of cytotoxicity is essential for design and construction of more effective polycations for nucleic acid delivery. A single toxicity assay cannot stand alone in determining the type and extent of damage or cell death mechanism. In this chapter we describe a lactate dehydrogenase (LDH) assay for high-throughput screening that can be used as a starting point for further detailed cytotoxicity determination. LDH release is considered an early event in necrosis but a late event in apoptosis. An accurate temporal assessment of the toxic responses is crucial as late apoptosis may convert into necrosis as well as in situations where cell death is initiated without any visible cell morphological changes or responses in assays measuring late events, resulting in early ongoing toxicity being overlooked.

Publication metadata

Author(s): Parhamifar L, Andersen H, Moghimi SM

Publication type: Book Chapter

Publication status: Published

Book Title: Nanotechnology for Nucleic Acid Delivery: Methods and Protocols

Year: 2019

Volume: 1943

Pages: 291-299

Online publication date: 06/03/2019

Acceptance date: 02/04/2018

Series Title: Methods in Molecular Biology

Publisher: Humana Press

Place Published: New York


DOI: 10.1007/978-1-4939-9092-4_18

PubMed id: 30838623

Library holdings: Search Newcastle University Library for this item

ISBN: 9781493990917