Browse by author
Lookup NU author(s): Professor Moein MoghimiORCiD
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
A better understanding of the molecular basis of polycation-mediated impairment of mitochondrial bioenergetics might improve the design and synthesis of more efficient and safer polymeric transfectants. Here we utilize the phosphorylation control protocol for studying the effect of polycations on mitochondrial respiration in intact mammalian cells using Oxygraph-2k (OROBOROS). The protocol offers an opportunity to comprehensively monitor mitochondrial respiration through consecutive additions of various cell membrane permeable compounds that alter mitochondrial respiration, thus providing useful information on different states of mitochondrial respiration. Furthermore, we demonstrate how to analyze the data obtained with the phosphorylation control protocol and how to calculate the respiratory flux ratios, which can be used as indicators of respiratory functionality and mitochondrial health.
Author(s): Hall A, Moghimi SM
Publication type: Book Chapter
Publication status: Published
Book Title: Nanotechnology for Nucleic Acid Delivery: Methods and Protocols
Online publication date: 06/03/2019
Acceptance date: 02/04/2018
Series Title: Methods in Molecular Biology
Publisher: Humana Press
Place Published: New York
PubMed id: 30838625
Library holdings: Search Newcastle University Library for this item