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Lookup NU author(s): Professor Che Connon,
Dr Ricardo Martins Gouveia
This is the authors' accepted manuscript of an article that has been published in its final definitive form by American Chemical Society, 2019.
For re-use rights please refer to the publisher's terms and conditions.
In this study, we used tissue templating technology to direct human dermal fibroblasts to biofabricate large-area tissues that closely emulate the natural dermis. This technology also allowed the new tissues to promote their own release from the template surface, thus facilitating their recovery as self-sustained, scaffold-free dermal equivalents solely comprising human cells and their own extracellular matrix. The structure and composition of these dermal self-lifting autogenous tissue equivalents (SLATEs) were evaluated in detail and were shown to closely correlate to normal tissue function. Specifically, dermal SLATEs were shown to be composed of a dense collagen-based matrix interwoven with dermal-characteristic elastic fibers. In addition, the mechanical properties of these tissues (i.e., robustness, elastic modulus, and resistance to contraction and enzymatic degradation) were comparable to those of the natural human dermis. Furthermore, dermal SLATEs were capable of constituting tissues with a higher-order complexity by serving as a substrate to support the growth of keratinocytes into stratified epithelia with distinct layers of differentiation. This work thus illustrates the great potential of tissue templating technologies and how these can pave the way for the biofabrication of easily retrievable, scaffold-free human skin tissues with a structure, composition, and function suitable for both clinical and nonclinical applications.
Author(s): Connon CJ, Gouveia RM
Publication type: Article
Publication status: Published
Journal: ACS Applied Bio Materials
Print publication date: 18/02/2019
Online publication date: 31/12/2018
Acceptance date: 30/12/2018
Date deposited: 01/04/2019
ISSN (electronic): 2576-6422
Publisher: American Chemical Society
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