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Array formatting of the heat-transfer method (HTM) for the detection of small organic molecules by molecularly imprinted polymers

Lookup NU author(s): Professor Marloes PeetersORCiD



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


In this work we present the first steps towards a molecularly imprinted polymer (MIP)-based biomimetic sensor array for the detection of small organic molecules via the heat-transfer method (HTM). HTM relies on the change in thermal resistance upon binding of the target molecule to the MIP-type receptor. A flow-through sensor cell was developed, which is segmented into four quadrants with a volume of 2.5 μL each, allowing four measurements to be done simultaneously on a single substrate. Verification measurements were conducted, in which all quadrants received a uniform treatment and all four channels exhibited a similar response. Subsequently, measurements were performed in quadrants, which were functionalized with different MIP particles. Each of these quadrants was exposed to the same buffer solution, spiked with different molecules, according to the MIP under analysis. With the flow cell design we could discriminate between similar small organic molecules and observed no significant cross-selectivity. Therefore, the MIP array sensor platform with HTM as a readout technique, has the potential to become a low-cost analysis tool for bioanalytical applications. © 2014 by the authors; licensee MDPI, Basel, Switzerland.

Publication metadata

Author(s): Wackers G, Vandenryt T, Cornelis P, Kellens E, Thoelen R, De Ceuninck W, Losada-Perez P, Van Grinsven B, Peeters M, Wagner P

Publication type: Article

Publication status: Published

Journal: Sensors

Year: 2014

Volume: 14

Issue: 6

Pages: 11016-11030

Online publication date: 20/06/2014

Acceptance date: 17/06/2014

Date deposited: 15/04/2019

ISSN (print): 1424-8239

ISSN (electronic): 1424-8220

Publisher: MDPI AG


DOI: 10.3390/s140611016

PubMed id: 24955945


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