Browse by author
Lookup NU author(s): Professor Marloes PeetersORCiD
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Molecularly imprinted polymers (MIPs) are synthetic receptors that are able to specifically bind their target molecules in complex samples, making them a versatile tool in biosensor technology. The combination of MIPs as a recognition element with quartz crystal microbalances (QCM-D with dissipation monitoring) gives a straightforward and sensitive device, which can simultaneously measure frequency and dissipation changes. In this work, bulk-polymerized l-nicotine MIPs were used to test the feasibility of l-nicotine detection in saliva and urine samples. First, l-nicotine-spiked saliva and urine were measured after dilution in demineralized water and 0.1× phosphate-buffered saline solution for proof-of-concept purposes. l-nicotine could indeed be detected specifically in the biologically relevant micromolar concentration range. After successfully testing on spiked samples, saliva was analyzed, which was collected during chewing of either nicotine tablets with different concentrations or of smokeless tobacco. The MIPs in combination with QCM-D were able to distinguish clearly between these samples: This proves the functioning of the concept with saliva, which mediates the oral uptake of nicotine as an alternative to the consumption of cigarettes. [Figure not available: see fulltext.] © 2013 Springer-Verlag Berlin Heidelberg.
Author(s): Alenus J, Ethirajan A, Horemans F, Weustenraed A, Csipai P, Gruber J, Peeters M, Cleij TJ, Wagner P
Publication type: Article
Publication status: Published
Journal: Analytical and Bioanalytical Chemistry
Year: 2013
Volume: 405
Issue: 20
Pages: 6479-6487
Print publication date: 01/08/2013
Online publication date: 11/06/2013
ISSN (print): 1618-2642
ISSN (electronic): 1618-2650
Publisher: Springer
URL: https://doi.org/10.1007/s00216-013-7080-1
DOI: 10.1007/s00216-013-7080-1
PubMed id: 23754330
Altmetrics provided by Altmetric
