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Lookup NU author(s): Dr Marloes PeetersORCiD
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Histamine is a biogenic amine that is indispensable in the efficient functioning of various physiological systems. In previous work, a molecularly imprinted polymer (MIP) based sensor platform with impedimetric read-out was presented which could rapidly and at low cost determine histamine concentrations in buffer solutions within pH 7-9.(1, 2) For diagnostic applications, histamine should be detectable in a wider pH range as it mostly occurs in mildly acidic environments. To understand this pH-dependent response of the MIP sensor, we propose a statistical binding analysis model. Within this model, we predict the theoretical performance of MIP based on acrylic acid in the required pH range and verify these results experimentally by UV-vis spectroscopy, microgravimetry, and impedance spectroscopy. Using impedimetric read-out, specific and selective detection of histamine in the physiologically relevant nanomolar concentration range is possible in neutral and mildly acidic phosphate buffer. Finally, this sensor platform was used to analyze the histamine concentration of mildly acidic bowel fluid samples of several test persons. We show that this sensor provides reliable data in the relevant concentration regime, which was validated independently by enzyme-linked immuno sorbent assay (ELISA) tests. © 2012 American Chemical Society.
Author(s): Peeters M, Troost FJ, Mingels RHG, Welsch T, Van Grinsven B, Vranken T, Ingebrandt S, Thoelen R, Cleij TJ, Wagner P
Publication type: Article
Publication status: Published
Journal: Analytical Chemistry
Print publication date: 05/02/2013
Online publication date: 18/12/2012
ISSN (print): 0003-2700
ISSN (electronic): 1520-6882
Publisher: American Chemical Society
PubMed id: 23249279
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