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Chromatin regulation by Histone H4 acetylation at Lysine 16 during cell death and differentiation in the myeloid compartment

Lookup NU author(s): Dr Daniel Rico RodriguezORCiD, Inmaculada Hernandez Lopez



This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).


© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.Histone H4 acetylation at Lysine 16 (H4K16ac) is a key epigenetic mark involved in gene regulation, DNA repair and chromatin remodeling, and though it is known to be essential for embryonic development, its role during adult life is still poorly understood. Here we show that this lysine is massively hyperacetylated in peripheral neutrophils. Genome-wide mapping of H4K16ac in terminally differentiated blood cells, along with functional experiments, supported a role for this histone post-translational modification in the regulation of cell differentiation and apoptosis in the hematopoietic system. Furthermore, in neutrophils, H4K16ac was enriched at specific DNA repeats. These DNA regions presented an accessible chromatin conformation and were associated with the cleavage sites that generate the 50 kb DNA fragments during the first stages of programmed cell death. Our results thus suggest that H4K16ac plays a dual role in myeloid cells as it not only regulates differentiation and apoptosis, but it also exhibits a non-canonical structural role in poising chromatin for cleavage at an early stage of neutrophil cell death.

Publication metadata

Author(s): Urdinguio RG, Lopez V, Bayon GF, Diaz de la Guardia R, Sierra MI, Garcia-Torano E, Perez RF, Garcia MG, Carella A, Pruneda PC, Prieto C, Dmitrijeva M, Santamarina P, Belmonte T, Mangas C, Diaconu E, Ferrero C, Tejedor JR, Fernandez-Morera JL, Bravo C, Bueno C, Sanjuan-Pla A, Rodriguez RM, Suarez-Alvarez B, Lopez-Larrea C, Bernal T, Colado E, Balbin M, Garcia-Suarez O, Chiara MD, Saenz-de-Santa-Maria I, Rodriguez F, Pando-Sandoval A, Rodrigo L, Santos L, Salas A, Vallejo-Diaz J, Carrera AC, Rico D, Hernandez-Lopez I, Vaya A, Ricart JM, Seto E, Sima-Teruel N, Vaquero A, Valledor L, Canal MJ, Pisano D, Grana-Castro O, Thomas T, Voss AK, Menendez P, Villar-Garea A, Deutzmann R, Fernandez AF, Fraga MF

Publication type: Article

Publication status: Published

Journal: Nucleic Acids Research

Year: 2019

Volume: 47

Issue: 10

Pages: 5016-5037

Print publication date: 04/06/2019

Online publication date: 29/03/2019

Acceptance date: 15/03/2019

Date deposited: 24/06/2019

ISSN (print): 1362-4954

ISSN (electronic): 1362-4962

Publisher: Oxford University Press


DOI: 10.1093/nar/gkz195

PubMed id: 30923829


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