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Structural Investigations of Protein–Lipid Complexes Using Neutron Scattering

Lookup NU author(s): Professor Jeremy LakeyORCiD


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© Springer Science+Business Media, LLC, part of Springer Nature 2019.Neutron scattering has significant benefits for examining the structure of protein–lipid complexes. Cold (slow) neutrons are nondamaging and predominantly interact with the atomic nucleus, meaning that neutron beams can penetrate deeply into samples, which allows for flexibility in the design of samples studied. Most importantly, there is a strong difference in neutron scattering length (i.e., scattering power) between protium (H11, 99.98% natural abundance) and deuterium (H12 or D, 0.015%). Through the mixing of H2O and D2O in the samples and in some cases the deuterium labeling of the biomolecules, components within a complex can be hidden or enhanced in the scattering signal. This enables both the overall structure and the relative distribution of components within a complex to be resolved. Lipid–protein complexes are most commonly studied using neutron reflectometry (NR) and small angle neutron scattering (SANS). In this review the methodologies to produce and examine a variety of model biological membrane systems using SANS and NR are detailed. These systems include supported lipid bilayers derived from vesicle dispersions or Langmuir–Blodgett deposition, tethered bilayer systems, membrane protein–lipid complexes and polymer wrapped lipid nanodiscs. The three key stages of any SANS/NR study on model membrane systems—sample preparation, data collection, and analysis—are described together with some background on the techniques themselves.

Publication metadata

Author(s): Clifton LA, Hall SCL, Mahmoudi N, Knowles TJ, Heinrich F, Lakey JH

Editor(s): Kleinschmidt, JH

Publication type: Book Chapter

Publication status: Published

Book Title: Lipid-Protein Interactions: Methods and Protocols

Year: 2019

Volume: 2003

Pages: 201-251

Online publication date: 20/06/2019

Acceptance date: 02/04/2019

Series Title: Methods in Molecular Biology

Publisher: Humana Press Inc.

Place Published: New York


DOI: 10.1007/978-1-4939-9512-7_11

Library holdings: Search Newcastle University Library for this item

ISBN: 9781493995110