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A prototype Antibody-based Biosensor for Measurement of Salivary MMP-8 in Periodontitis using Surface Acoustic Wave technology

Lookup NU author(s): Dr John TaylorORCiD, Dr Susan BissettORCiD, Dr Deepan Shah, Professor Philip Preshaw



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Periodontitis is an economically important disease which is highly prevalent worldwide. current diagnostic approaches are time-consuming and require interpretation of multiple aspects of clinical and radiographic assessment. Chair-side monitoring of inflammatory mediators of periodontitis could provide immediate information about disease activity, which can inform patient management. We aimed to develop a novel prototype biosensor to measure salivary matrix metalloproteinase-8 (MMP-8) using specific antibodies and surface acoustic wave (SAW) technology. The analytical performance of the prototype biosensor was compared to standard enzyme-linked immunosorbent assay (ELISA) using unstimulated saliva samples obtained from patients with periodontitis before and after non-surgical treatment (N = 58), patients with gingivitis (N = 54) and periodontally healthy volunteers (N = 65). Receiver operator characteristic (ROC) analysis for distinguishing periodontitis from health revealed an almost identical performance between the sensor and ELISA assays (area under curve values (AUC): ELISA 0.93; SAW 0.89). Furthermore, both analytical approaches yielded readouts which distinguished between heath, gingivitis and periodontitis, correlated identically with clinical measures of periodontal disease and recorded similar post-treatment decreases in salivary MMP-8 in periodontitis. The assay time for our prototype device is 20 minutes. The prototype SAW biosensor is a novel and rapid method of monitoring periodontitis which delivers similar analytical performance to conventional laboratory assays.

Publication metadata

Author(s): Taylor JJ, Jaedicke KJ, vandeMerwe RC, Bissett SM, Landsdowne N, Whall KM, Pickering K, Thornton V, Lawson V, Yatsuda H, Kogai T, Shah D, Athey D, Preshaw PM

Publication type: Article

Publication status: Published

Journal: Scientific Reports

Year: 2019

Volume: 9

Print publication date: 30/07/2019

Online publication date: 30/07/2019

Acceptance date: 11/07/2019

Date deposited: 31/07/2019

ISSN (electronic): 2045-2322

Publisher: Springer Nature Publishing


DOI: 10.1038/s41598-019-47513-w


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