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Lookup NU author(s): Dr Jean Franco Castro Figueroa, Dr Imen Nouioui, Professor Alan Bull, Professor Michael Goodfellow
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2019, The Author(s).A set of oligonucleotide primers, Rubro223f and Rubro454r, were found to amplify a 267 nucleotide sequence of 16S rRNA genes of Rubrobacter type strains. The primers distinguished members of this genus from other deeply-rooted actinobacterial lineages corresponding to the genera Conexibacter, Gaiella, Parviterribacter, Patulibacter, Solirubrobacter and Thermoleophilum of the class Thermoleophilia. Amplification of DNA bands of about 267 nucleotides were generated from environmental DNA extracted from soil samples taken from two locations in the Atacama Desert. Sequencing of a DNA library prepared from the bands showed that all of the clones fell within the evolutionary radiation occupied by the genus Rubrobacter. Most of the clones were assigned to two lineages that were well separated from phyletic lines composed of Rubrobacter type strains. It can be concluded that primers Rubro223f and Rubro454r are specific for the genus Rubrobacter and can be used to detect the presence and abundance of members of this genus in the Atacama Desert and other biomes.
Author(s): Castro JF, Nouioui I, Asenjo JA, Andrews B, Bull AT, Goodfellow M
Publication type: Article
Publication status: Published
Journal: Antonie van Leeuwenhoek
Year: 2019
Volume: 112
Pages: 1863-1874
Online publication date: 12/08/2019
Acceptance date: 01/08/2019
Date deposited: 30/08/2019
ISSN (print): 0003-6072
ISSN (electronic): 1572-9699
Publisher: Springer Netherlands
URL: https://doi.org/10.1007/s10482-019-01314-3
DOI: 10.1007/s10482-019-01314-3
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