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Evaluation of a novel rapidly-growing mycobacteria medium for isolation of Mycobacterium abscessus complex from respiratory specimens from patients with bronchiectasis

Lookup NU author(s): Dr John Perry



This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).


© 2019This single center study assessed the performance of a novel solid rapidly-growing mycobacteria (RGM) medium for the recovery of nontuberculous mycobacteria (NTM), especially Mycobacterium abscessus complex, in patients with underlying bronchiectasis. A total of 297 mycobacterial sputa from 116 patients were plated directly on RGM medium and, following decontamination, onto an agar biplate [Middlebrook 7H11 and Mitchison (selective) agar] and into broth media (VersaTrek). The recovery of M. abscessus complex was increased by approximately 12% by implementation of the RGM medium. Contamination was reduced to 2% from 48% and 95% on routine solid media and broth cultures respectively. Our study corroborated previous studies in that recovery of M. abscessus complex was enhanced and contamination was virtually eliminated without the need for specimen decontamination when utilizing RGM medium.© 2019Microbiology; Mycobacteria; Mycobacterium abscessus; Rapidly growing mycobacteria; Mycobacterial culture; Cystic fibrosis.

Publication metadata

Author(s): Brown-Elliott BA, Molina S, Fly T, Njie O, Stribley P, Stephenson D, Wallace RJ, Perry JD

Publication type: Article

Publication status: Published

Journal: Heliyon

Year: 2019

Volume: 5

Issue: 10

Online publication date: 24/10/2019

Acceptance date: 15/10/2019

Date deposited: 04/11/2019

ISSN (electronic): 2405-8440

Publisher: Elsevier Ltd


DOI: 10.1016/j.heliyon.2019.e02684


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Funder referenceFunder name
bioMerieux for the development and evaluation of diagnostic products
University of TX Health Science Center at Tyler