Toggle Main Menu Toggle Search

Open Access padlockePrints

Microfluidic time-lapse analysis and reevaluation of the Bacillus subtilis cell cycle

Lookup NU author(s): Dr Seoungjun Lee, Dr Ling Juan Wu, Professor Jeff ErringtonORCiD



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


© 2019 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.Recent studies taking advantage of automated single-cell time-lapse analysis have reignited interest in the bacterial cell cycle. Several studies have highlighted alternative models, such as Sizer and Adder, which differ essentially in relation to whether cells can measure their present size or their amount of growth since birth. Most of the recent work has been done with Escherichia coli. We set out to study the well-characterized Gram-positive bacterium, Bacillus subtilis, at the single-cell level, using an accurate fluorescent marker for division as well as a marker for completion of chromosome replication. Our results are consistent with the Adder model in both fast and slow growth conditions tested, and with Sizer but only at the slower growth rate. We also find that cell size variation arises not only from the expected variation in size at division but also that division site offset from mid-cell contributes to a significant degree. Finally, although traditional cell cycle models imply a strong connection between the termination of a round of replication and subsequent division, we find that at the single-cell level these events are largely disconnected.

Publication metadata

Author(s): Lee S, Wu LJ, Errington J

Publication type: Article

Publication status: Published

Journal: MicrobiologyOpen

Year: 2019

Volume: 8

Issue: 10

Online publication date: 13/06/2019

Acceptance date: 13/05/2019

Date deposited: 04/11/2019

ISSN (electronic): 2045-8827

Publisher: John Wiley & Sons Ltd.


DOI: 10.1002/mbo3.876

PubMed id: 31197963


Altmetrics provided by Altmetric


Funder referenceFunder name