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Lookup NU author(s): Dr Ana Lorena Morales Garcia,
Professor Mark Geoghegan
This is the authors' accepted manuscript of an article that has been published in its final definitive form by American Chemical Society, 2019.
For re-use rights please refer to the publisher's terms and conditions.
© 2019 American Chemical Society.Force spectroscopy was used to show that extracellular DNA (eDNA) has a pre-eminent structural role in a biofilm. The adhesive behavior of extracellular polymeric substances to poly(ethylene terephthalate), a model hydrophobic surface, was measured in response to their degradation by hydrolytic enzymes known for their biofilm dispersion potential: DNaseI, protease, cellulase, and mannanase. Only treatment with DNaseI significantly decreased the adhesive force of the model bacterium Micrococcus luteus with the surface, and furthermore this treatment almost completely eliminated any components of the biofilm maintaining the adhesion, establishing a key structural role for eDNA.
Author(s): Blakeman JT, Morales-Garcia AL, Mukherjee J, Gori K, Hayward AS, Lant NJ, Geoghegan M
Publication type: Article
Publication status: Published
Online publication date: 17/04/2019
Acceptance date: 02/04/2019
Date deposited: 10/11/2020
ISSN (print): 0743-7463
ISSN (electronic): 1520-5827
Publisher: American Chemical Society
PubMed id: 30995049
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