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Lookup NU author(s): Dr Meryl Lusher
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Monoclonal antibodies were prepared against pancreatic stone protein, a protein which inhibits calcium carbonate precipitation. Two monoclonal antibodies designated D4 and 2E7 were characterized. Immunoadsorbant columns, obtained by linkage of these monoclonal antibodies to Affigel 10, have been used to isolate immunoreactive forms of pancreatic stone protein from nonactivated human pancreatic juice. These monoclonal antibodies permitted us to test the possible immunological relationship between pancreatic stone protein and human trypsin 1. No immunological similarity was found, in agreement with our previous results, and it was established that pancreatic stone protein is a novel protein and not a degradation product of human trypsin(ogen) 1.
Author(s): Provansal-Cheylan M, Lusher ME, Caro DeA, Multigner L, Montalto G, Sarles H, Delaage M
Publication type: Article
Publication status: Published
Journal: Biochimie
Year: 1986
Volume: 68
Issue: 9
Pages: 1109-1113
ISSN (print): 0300-9084
ISSN (electronic): 1638-6183
Publisher: Elsevier Masson
URL: http://dx.doi.org/10.1016/S0300-9084(86)80185-7
DOI: 10.1016/S0300-9084(86)80185-7
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