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Cholinergic signalling mechanisms and early implant healing phases in healthy versus generalized aggressive periodontitis patients: A prospective, case-control study

Lookup NU author(s): Dr Christopher NileORCiD



This is the authors' accepted manuscript of an article that has been published in its final definitive form by Blackwell Munksgaard, 2019.

For re-use rights please refer to the publisher's terms and conditions.


© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. Aims: Periodontal diseases negatively affect implant osseointegration. Perturbations in non-neuronal cholinergic signalling mechanisms are associated with periodontitis; however, their role in generalized aggressive periodontitis (GAgP) is unknown. The aim of this prospective case–control study was to determine the relationship between non-neuronal cholinergic signalling mechanisms, secreted Ly-6/uPAR-related protein-1 (SLURP-1), interleukin-17 (IL-17) family cytokines and healing of dental implants in health and GAgP. Material and Methods: Thirteen GAgP patients and seven periodontally healthy individuals (PH) were recruited. Peri-implant crevicular fluid (PICF) was obtained at baseline and 1 month post-placement. Acetylcholine (ACh) levels and cholinesterase activity were determined biochemically. SLURP-1, IL-17A and IL-17E levels were determined by ELISA. Marginal bone loss (MBL) at 1 and 6 months post-placement was determined radiographically. Results: The concentration of ACh, cholinesterase activity and IL-17A levels was elevated in PICF of patients with GAgP compared to PH individuals at baseline and 1 month post-placement. The concentration of ACh and cholinesterase activity levels in PICF correlated with levels of IL-17A and MBL around implants 1 month post-placement in patients with GAgP. Conclusions: Non-neuronal cholinergic mechanisms may play a role in the aetiopathogenesis of GAgP and may directly or indirectly, through modulation of IL-17A, influence early implant osseointegration and potential long-term implant survival.

Publication metadata

Author(s): Meric P, Buduneli N, Kanmaz B, Gurlek O, Comlekoglu E, Calvert G, Lappin DF, Nile C

Publication type: Article

Publication status: Published

Journal: Journal of Clinical Periodontology

Year: 2019

Volume: 46

Issue: 11

Pages: 1155-1163

Print publication date: 01/11/2019

Online publication date: 24/08/2019

Acceptance date: 21/08/2019

Date deposited: 21/10/2020

ISSN (print): 0303-6979

ISSN (electronic): 1600-051X

Publisher: Blackwell Munksgaard


DOI: 10.1111/jcpe.13185

PubMed id: 31444906


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