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Lookup NU author(s): Dr Christopher NileORCiD
This is the authors' accepted manuscript of an article that has been published in its final definitive form by Blackwell Munksgaard, 2018.
For re-use rights please refer to the publisher's terms and conditions.
© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons LtdAim: The oral mucosa possesses a non-neuronal cholinergic system. This study aimed to determine clinical evidence for a role of cholinergic mechanisms in the pathogenesis of periodontal diseases. Materials and Methods: Fifty healthy participants, 52 patients with gingivitis and 49 with periodontitis were recruited. Full periodontal parameters were recorded and saliva and gingival crevicular fluid (GCF) collected. Levels of acetylcholine and inflammatory mediators were quantified using commercially available assay kits. Acetylcholinesterase and butyrylcholinesterase activities were measured using a published biochemical assay. Results: Acetylcholine levels are significantly elevated in saliva and GCF, whereas GCF levels of butyrylcholinesterase activity are significantly decreased, in patients with periodontal diseases. Acetylcholine levels in saliva and GCF correlated positively with clinical markers of disease severity and with increased levels of IL-17A and IL-17F. In contrast, butyrylcholinesterase activity levels in GCF showed significant negative correlations with clinical markers of disease severity and IL-17A and IL-17F levels. None of the findings were due to smoking. Conclusions: Elevated acetylcholine levels and reduced butyrylcholinesterase activity are clinically associated with periodontal diseases and elevated levels of IL-17A and IL-17F. Therefore, non-neuronal cholinergic mechanisms may influence IL-17 biology and the aetiopathogenesis of periodontal diseases and therefore are possible therapeutic targets.
Author(s): Apatzidou DA, Iskas A, Konstantinidis A, Alghamdi AM, Tumelty M, Lappin DF, Nile CJ
Publication type: Article
Publication status: Published
Journal: Journal of Clinical Periodontology
Print publication date: 01/10/2018
Online publication date: 19/07/2018
Acceptance date: 16/07/2018
Date deposited: 24/02/2020
ISSN (print): 0303-6979
ISSN (electronic): 1600-051X
Publisher: Blackwell Munksgaard
PubMed id: 30022504
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