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Stem cell-like populations and immunoregulatory molecules in periodontal granulation tissue

Lookup NU author(s): Dr Christopher NileORCiD



This is the authors' accepted manuscript of an article that has been published in its final definitive form by Blackwell Munksgaard, 2018.

For re-use rights please refer to the publisher's terms and conditions.


© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons LtdBackground and Objectives: Determine the presence of mesenchymal stem cells (MSCs) in healthy periodontal tissue and periodontal granulation tissue (GT) and explore associations between immuno-regulatory molecules and selected subgingival microorganisms. Material and Methods: Mesenchymal stem cells were isolated, propagated and characterised by flow cytometry from a region of healthy gingival tissue and inflamed GT of 10 systemically healthy non-smokers with chronic periodontitis. Tissue levels of immunoregulatory molecules were determined by qPCR and Gingival Crevicular Fluid (GCF) levels by ELISA. Subgingival plaque levels of periodontal pathogens were determined by qPCR. Results: Cells with MSC-properties were isolated from both inflamed GT and healthy gingival (G) tissue. A pro-inflammatory process predominated in GT which was partly reflected in GCF and putative periodontal pathogens were higher at diseased sites. However, there was no significant difference in surface levels of mesenchymal (CD90, CD73, CD146, CD271, STRO-1), endothelial (CD105, CD106), hematopoietic (CD34, CD45) and embryonic (SSEA-4) stem cell markers between MSCs isolated from GT and G tissue. Conclusion: Periodontal lesions, albeit inflamed, retain healing potential as inferred by the presence of MSC-like cells with similar immunophenotypic characteristics to those found in healthy periodontal tissue. Therefore, there might be merits for healing in preserving sufficient GT in-situ during periodontal surgery.

Publication metadata

Author(s): Apatzidou DA, Nile C, Bakopoulou A, Konstantinidis A, Lappin DF

Publication type: Article

Publication status: Published

Journal: Journal of Periodontal Research

Year: 2018

Volume: 53

Issue: 4

Pages: 610-621

Print publication date: 10/07/2018

Online publication date: 23/04/2018

Acceptance date: 22/02/2018

Date deposited: 24/02/2020

ISSN (print): 0022-3484

ISSN (electronic): 1600-0765

Publisher: Blackwell Munksgaard


DOI: 10.1111/jre.12551

PubMed id: 29687448


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