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Lookup NU author(s): Dr Adam WollmanORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2019 The AuthorsHow genomic DNA is organized in the nucleus is a long-standing question. We describe a single-molecule bioimaging method utilizing super-localization precision coupled to fully quantitative image analysis tools, towards determining snapshots of parts of the 3D genome architecture of model eukaryote budding yeast Saccharomyces cerevisiae with exceptional millisecond time resolution. We employ astigmatism imaging to enable robust extraction of 3D position data on genomically encoded fluorescent protein reporters that bind to DNA. Our relatively straightforward method enables snippets of 3D architectures of likely single genome conformations to be resolved captured via DNA-sequence specific binding proteins in single functional living cells.
Author(s): Wollman AJM, Hedlund EG, Shashkova S, Leake MC
Publication type: Article
Publication status: Published
Journal: Methods
Year: 2020
Volume: 170
Pages: 82-89
Print publication date: 01/01/2020
Online publication date: 26/06/2019
Acceptance date: 22/06/2019
Date deposited: 10/02/2020
ISSN (print): 1046-2023
ISSN (electronic): 1095-9130
Publisher: Academic Press Inc.
URL: https://doi.org/10.1016/j.ymeth.2019.06.021
DOI: 10.1016/j.ymeth.2019.06.021
PubMed id: 31252059
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