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Lookup NU author(s): Dr Adam WollmanORCiD
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© Springer International Publishing Switzerland 2016. The method of action of many antibiotics is to interfere with DNA replication—quinolones trap DNA gyrase and topoisomerase proteins onto DNA while metronidazole causes single- and double-stranded breaks in DNA. To understand how bacteria respond to these drugs, it is important to understand the repair processes utilised when DNA replication is blocked. We have used tandem lac operators inserted into the chromosome bound by fluorescently labelled lac repressors as a model protein block to replication in E. coli. We have used dual-colour, alternating-laser, single-molecule narrowfield microscopy to quantify the amount of operator at the block and simultaneously image fluorescently labelled DNA polymerase. We anticipate use of this system as a quantitative platform to study replication stalling and repair proteins.
Author(s): Wollman AJM, Syeda AH, McGlynn P, Leake MC
Editor(s): Mark C. Leake
Publication type: Book Chapter
Publication status: Published
Book Title: Biophysics of Infection
Year: 2016
Volume: 915
Pages: 5-16
Online publication date: 19/05/2016
Acceptance date: 02/04/2016
Series Title: Advances in Experimental Medicine and Biology
Publisher: Springer
Place Published: New York
URL: https://doi.org/10.1007/978-3-319-32189-9_2
DOI: 10.1007/978-3-319-32189-9_2
PubMed id: 27193534
Library holdings: Search Newcastle University Library for this item
ISBN: 9783319321899