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Single-molecule observation of DNA replication repair pathways in E. coli

Lookup NU author(s): Dr Adam WollmanORCiD


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© Springer International Publishing Switzerland 2016. The method of action of many antibiotics is to interfere with DNA replication—quinolones trap DNA gyrase and topoisomerase proteins onto DNA while metronidazole causes single- and double-stranded breaks in DNA. To understand how bacteria respond to these drugs, it is important to understand the repair processes utilised when DNA replication is blocked. We have used tandem lac operators inserted into the chromosome bound by fluorescently labelled lac repressors as a model protein block to replication in E. coli. We have used dual-colour, alternating-laser, single-molecule narrowfield microscopy to quantify the amount of operator at the block and simultaneously image fluorescently labelled DNA polymerase. We anticipate use of this system as a quantitative platform to study replication stalling and repair proteins.

Publication metadata

Author(s): Wollman AJM, Syeda AH, McGlynn P, Leake MC

Editor(s): Mark C. Leake

Publication type: Book Chapter

Publication status: Published

Book Title: Biophysics of Infection

Year: 2016

Volume: 915

Pages: 5-16

Online publication date: 19/05/2016

Acceptance date: 02/04/2016

Series Title: Advances in Experimental Medicine and Biology

Publisher: Springer

Place Published: New York


DOI: 10.1007/978-3-319-32189-9_2

PubMed id: 27193534

Library holdings: Search Newcastle University Library for this item

ISBN: 9783319321899