Browse by author
Lookup NU author(s): Dr Adam WollmanORCiD
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
© The Authors Journal compilation ©2015 Biochemical Society. DNA-interacting proteins have roles in multiple processes, many operating as molecular machines which undergo dynamic meta-stable transitions to bring about their biological function. To fully understand this molecular heterogeneity, DNA and the proteins that bind to it must ideally be interrogated at a single molecule level in their native in vivo environments, in a time-resolved manner, fast enough to sample the molecular transitions across the free-energy landscape. Progress has been made over the past decade in utilizing cutting-edge tools of the physical sciences to address challenging biological questions concerning the function and modes of action of several different proteins which bind to DNA. These physiologically relevant assays are technically challenging but can be complemented by powerful and often more tractable in vitro experiments which confer advantages of the chemical environment with enhanced detection signal-to-noise of molecular signatures and transition events. In the present paper, we discuss a range of techniques we have developed to monitor DNA-protein interactions in vivo, in vitro and in silico. These include bespoke single-molecule fluorescence microscopy techniques to elucidate the architecture and dynamics of the bacterial replisome and the structural maintenance of bacterial chromosomes, as well as new computational tools to extract single-molecule molecular signatures from live cells to monitor stoichiometry, spatial localization and mobility in living cells. We also discuss recent developments from our laboratory made in vitro, complementing these in vivo studies, which combine optical and magnetic tweezers to manipulate and image single molecules of DNA, with and without bound protein, in a new super-resolution fluorescence microscope.
Author(s): Wollman AJM, Miller H, Zhou Z, Leake MC
Publication type: Article
Publication status: Published
Journal: Biochemical Society Transactions
Print publication date: 01/04/2015
Acceptance date: 19/09/2014
ISSN (print): 0300-5127
ISSN (electronic): 1470-8752
Publisher: Portland Press Ltd
PubMed id: 26020443
Altmetrics provided by Altmetric