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Lookup NU author(s): Dr Chien-Yi ChangORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2016 Chan et al.Functional genomics research can give us valuable insights into bacterial gene function. RNA Sequencing (RNA-seq) can generate information on transcript abundance in bacteria following abiotic stress treatments. In this study, we used the RNA-seq technique to study the transcriptomes of the opportunistic nosocomial pathogen Pseudomonas aeruginosa PAO1 following heat shock. Samples were grown at both the human body temperature (37 ° C) and an arbitrarily-selected temperature of 46 ° C. In this work using RNA-seq, we identified 133 genes that are differentially expressed at 46 ° C compared to the human body temperature. Our work identifies some key P. aeruginosa PAO1 genes whose products have importance in both environmental adaptation as well as in vivo infection in febrile hosts. More importantly, our transcriptomic results show that many genes are only expressed when subjected to heat shock. Because the RNA-seq can generate high throughput gene expression profiles, our work reveals many unanticipated genes with further work to be done exploring such genes products.
Author(s): Chan K-G, Priya K, Chang C-Y, Abdul Rahman AY, Tee KK, Yin W-F
Publication type: Article
Publication status: Published
Online publication date: 19/07/2016
Acceptance date: 15/06/2016
Date deposited: 25/02/2020
ISSN (electronic): 2167-8359
Publisher: PeerJ Ltd
PubMed id: 27547539
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