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A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

Lookup NU author(s): Professor Yen Nee Tan

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Abstract

A protocol for small molecular drug screening based on in-situ synthesis of ultrasmall fluorescent gold nanoclusters (Au NCs) using drug-loaded protein as template is presented. This method is simple to determine the binding affinity of drugs to a target protein by a visible fluorescent signal emitted from the protein-templated Au NCs.

Publication metadata

Author(s): Yu Y, New S, Lin J, Su X, Tan YN

Publication type: Article

Publication status: Published

Journal: Journal of Visualized Experiments

Year: 2015

Issue: 104

Online publication date: 16/10/2015

Acceptance date: 16/10/2015

ISSN (electronic): 1940-087X

Publisher: Journal of Visualized Experiments

URL: https://doi.org/10.3791/53261

DOI: 10.3791/53261

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