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Lookup NU author(s): Professor Yen Nee Tan
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
We have developed a unique DNA-assembled gold nanoparticles (AuNPs) dimer for dynamic light scattering (DLS) sensing of transcription factors, exemplified by estrogen receptor (ER) that binds specifically to a double-stranded (ds) DNA sequence containing estrogen response element (ERE). Here, ERE sequence is incorporated into the DNA linkers to bridge the AuNPs dimer for ER binding. Coupled with DLS, this AuNP dimer-based DLS detection system gave distinct readout of a single ‘complex peak’ in the presence of the target molecule (i.e., ER). This unique signature marked the first time that such nanostructures can be used to study transcription factor-DNA interactions, which DLS alone cannot do. This was also unlike previously reported AuNP-DLS assays that gave random and broad distribution of particles size upon target binding. In addition, the ERE-containing AuNP dimers could also suppress the light-scattering signal from the unbound proteins and other interfering factors (e.g., buffer background) and has potential for sensitive detection of target proteins in complex biological samples such as cell lysates. In short, the as-developed AuNP dimer probe coupled with DLS is a simple (mix and test), rapid (readout in ~5 min) and sensitive (low nM levels of ER) platform to detect sequence-specific protein-DNA binding event.
Author(s): Seow N, Tan YN, Yung LLY, Su X
Publication type: Article
Publication status: Published
Journal: Scientific Reports
Year: 2016
Volume: 5
Print publication date: 18/12/2015
Online publication date: 18/12/2015
Acceptance date: 13/10/2015
Date deposited: 21/03/2020
ISSN (electronic): 2045-2322
Publisher: Nature
URL: https://doi.org/10.1038/srep18293
DOI: 10.1038/srep18293
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