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Frankia soli sp. nov., an actinobacterium isolated from soil beneath ceanothus jepsonii

Lookup NU author(s): Dr Imen Nouioui

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Abstract

© 2020 The Authors.Actinobacterial strain CjT was directly isolated from soil beneath Ceanothus jepsonii growing in the USA. The strain formed cell structures typical of the genus Frankia including extensive hyphae, vesicles and sporangia, and it effectively nodulated members of the actinorhizal Colletieae, Elaeagnaceae and Myricaceae. The whole-cell hydrolysate of strain CjT was rich in meso-diaminopimelic acid and galactose, glucose, mannose, xylose, ribose and a trace of rhamnose. Tbe polar lipid profile contained phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol and glycophospholipid. The menaquinone was predominantly MK-9(H4). The fatty acid profile predominantly consisted of C17:1 ω8c, iso-C16:0, C15:0, C16:0 and C17:0. A multilocus sequence analysis phylogeny based on atp1, ftsZ, dnaK, gyrA and secA gene sequences positioned the strain within Elae-agnaceae-and Colletieae-nodulating species together with Frankia elaeagni DSM 46783T, Frankia discariae DSM 46785T and Frankia irregularis DSM 45899T. Pairwise 16S rRNA gene sequence similarities showed that strain CjT was most closely related to F. discariae DSM 46785T (99.78%) while their digital DNA–DNA hybridization value was 41.1%. Based on the overall analyses, strain CjT (=DSM 100623T=CECT 9041T) warrants classification as the type strain of a novel species, for which the name Frankia soli sp. nov. is proposed.

Publication metadata

Author(s): Gtari M, Ghodhbane-Gtari F, Nouioui I

Publication type: Article

Publication status: Published

Journal: International Journal of Systematic and Evolutionary Microbiology

Year: 2020

Volume: 70

Issue: 2

Pages: 1203-1209

Online publication date: 12/12/2019

Acceptance date: 15/11/2019

ISSN (print): 1466-5026

ISSN (electronic): 1466-5034

Publisher: Microbiology Society

URL: https://doi.org/10.1099/ijsem.0.003899

DOI: 10.1099/ijsem.0.003899

PubMed id: 31829914

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