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Linking Engineered Cells to Their Digital Twins: A Version Control System for Strain Engineering

Lookup NU author(s): Jonathan Tellechea Luzardo, Dr Charles WinterhalterORCiD, Dr PaweĊ‚ Widera, Dr Jurek Kozyra, Professor Natalio KrasnogorORCiD



This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).


As DNA sequencing and synthesis become cheaper and more easily accessible, the scale and complexity of biological engineering projects is set to grow. Yet, although there is an accelerating convergence between biotechnology and digital technology, a deficit in software and laboratory techniques diminishes the ability to make biotechnology more agile, reproducible, and transparent while, at the same time, limiting the security and safety of synthetic biology constructs. To partially address some of these problems, this paper presents an approach for physically linking engineered cells to their digital footprint-we called it digital twinning. This enables the tracking of the entire engineering history of a cell line in a specialized version control system for collaborative strain engineering via simple barcoding protocols.

Publication metadata

Author(s): Tellechea-Luzardo J, Winterhalter C, Widera P, Kozyra J, de Lorenzo V, Krasnogor N

Publication type: Article

Publication status: Published

Journal: ACS synthetic biology

Year: 2020

Volume: 9

Issue: 3

Pages: 536-545

Print publication date: 20/03/2020

Online publication date: 20/02/2020

Acceptance date: 01/10/2019

Date deposited: 23/11/2020

ISSN (electronic): 2161-5063

Publisher: American Chemical Society


DOI: 10.1021/acssynbio.9b00400

PubMed id: 32078768


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