Toggle Main Menu Toggle Search

Open Access padlockePrints

Multi-laboratory Validation of a New Marine Biodegradation Screening Test for Chemical Persistence Assessment

Lookup NU author(s): Dr Amelie Ott, Dr Tim Martin, Dr Kishor AcharyaORCiD, Professor Jason Snape, Professor Russell DavenportORCiD


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Current biodegradation screening tests are not specifically designed for persistence assessment of chemicals, often show high inter- and intra-test variability, and often give false negative biodegradation results. Based on previous studies and recommendations, an international ring test involving 13 laboratories validated a new test method for marine biodegradation with a focus on improving the reliability of screening to determine the environmental degradation potential of chemicals. The new method incorporated increased bacterial cell concentrations to better represent the microbial diversity; a chemical is likely to be exposed in the sampled environments and ran beyond 60 days, which is the half-life threshold for chemical persistence in the marine environment. The new test provided a more reliable and less variable characterization of the biodegradation behavior of five reference chemicals (sodium benzoate, triethanolamine, 4-nitrophenol, anionic polyacrylamide, and pentachlorophenol), with respect to REACH and OSPAR persistence thresholds, than the current OECD 306 test. The proposed new method provides a cost-effective screening test for non-persistence that could streamline chemical regulation and reduce the cost and animal welfare implications of further higher tier testing.

Publication metadata

Author(s): Ott A, Martin TJ, Acharya K, Lyon DY, Robinson N, Rowles B, Snape JR, Still I, Whale GF, Albright VC, Baverback P, Best N, Commander R, Eickhoff C, Finn S, Hidding B, Maischak H, Sowders KA, Taruki M, Walton HE, Wennberg AC, Davenport RJ

Publication type: Article

Publication status: Published

Journal: Environmental Science & Technology

Year: 2020

Volume: 54

Issue: 7

Pages: 4210-4220

Print publication date: 07/04/2020

Online publication date: 12/03/2020

Acceptance date: 12/03/2020

ISSN (print): 0013-936X

ISSN (electronic): 1520-5851

Publisher: American Chemical Society


DOI: 10.1021/acs.est.9b07710

PubMed id: 32162906


Altmetrics provided by Altmetric